【摘要】 为提高樟树籽仁油的生理效应和经济价值，需设法调整其链脂肪酸组成、降低其月桂酸含量。本研究分别以辛酸/癸酸为酰基供体，采用酶促酰基交换法进行反应，筛选出催化效果最佳的脂肪酶，并以月桂酸含量为评价指标优化低月桂酸樟树籽仁油制备工艺。结果表明：Lipozyme RM IM脂肪酶的催化性能最佳。采用Lipozyme RM IM脂肪酶得到癸酸反应的最佳条件为：底物癸酸与樟树籽仁油的物质的量之比为10：1，反应时间为6 h，反应温度为50℃，加酶量为总底物质量的6%，该条件下所得产品的中链脂肪酸质量分数分别为月桂酸11.40%，癸酸85.72%，辛酸0.24%。采用Lipozyme RM IM脂肪酶得到辛酸反应的最佳条件为：底物辛酸与樟树籽仁油的物质的量之比为6：1，反应温度为50℃，反应时间为6 h，加酶量为总底物质量的4%，该条件下所得产品的中链脂肪酸质量分数分别为月桂酸16.34%，癸酸54.53%，辛酸25.81%。该酶促酰基交换技术制备的低月桂酸樟树籽仁油产品的理化性质符合GB 2716—2018，可大幅提高樟树籽仁油的生理效应和经济价值。更多还原

【Abstract】 To further improve the physiological effect and economic value of CCSKO, it was necessary to reduce its lauric acid content. This study used caprylic acid/capric acid as acyl donors, and enzymatic acyl exchange method was used to screen out the lipase with the best catalytic effect. The preparation process of CCSKO with low lauric acid content was optimized by using lauric acid content as the evaluation index. The results showed that Lipozyme RM IM lipase had the best catalytic properties. The optimal conditions of capric acid acyl exchange reaction were as follows: the molar ratio of substrate capric acid to CCSKO was 10 ： 1, reaction time was 6 h, reaction temperature was 50 ℃, and enzyme load was 6% of the total substrate mass. Under these conditions, the mass fractions of medium-chain fatty acids was 11.40% lauric acid, 85.72% capric acid, and 0.24% caprylic acid. The optimal conditions of caprylic acid acyl exchange reaction were as follows: the molar ratio of substrate caprylic acid to CCSKO was 6 ： 1, reaction temperature was 50 ℃, reaction time was 6 h, and enzyme load was 4% of the total substrate mass. Under these conditions, the mass fractions of medium-chain fatty acids was 16.34% lauric acid, 54.53% capric acid, and 25.81% caprylic acid. The physicochemical properties of CCSKO with low lauric acid content prepared by the enzymatic acyl exchange technology conformed to the national standard GB 2716—2018, which could greatly improve the physiological effect and economic value of CCSKO.更多还原