【摘要】 目的 探讨高脂饮食干预小鼠内脏脂肪组织中恒定自然杀伤（iNK）T细胞和血脂的变化，分析iNK T细胞与脂代谢的相关性。方法 随机选取C57BL-6小鼠52只作为高脂饮食组和51只C57BL-6小鼠作为正常对照组，饮食干预12周，分别于第1、4、8、12周取两组小鼠附睾及肾周脂肪并进行称重，记录内脏脂肪重量（VFM），计算体脂肪含量（BFC）变化。流式细胞仪及激光扫描共聚焦显微镜检测内脏脂肪组织中iNK T细胞变化，全自动生化分析仪检测小鼠血脂水平，分析内脏脂肪组织中iNK T细胞与VFM、BFC及血脂的相关性。结果 与正常对照组比较，高脂饮食组干预12周，小鼠体质量、VFM、BFC、血清总胆固醇（TC）、三酰甘油（TG）、高密度脂蛋白胆固醇（HDL-C）显著升高，内脏脂肪组织中iNK T细胞含量明显下降（P<0.01）,血清低密度脂蛋白胆固醇（LDL-C）未见显著升高（P>0.05）。小鼠内脏脂肪组织中的iNK T细胞含量与VFM、BFC、血清HDL-C、TG呈负相关（r=-0.293、-0289、-0.337、-0.199,P<0.05）；与血清TC、LDL-C无相关性（r=-0.122、-0.082,P>0.05）。结论 高脂饮食小鼠VFM增加，脂肪组织中iNK T细胞含量降低；iNK T细胞数量与VFM、BFC、血清HDL-C、TG呈负相关。更多还原

【Abstract】 Objective To observe the changes of invariant natural killer T(iNK T) cells in visceral adipose tissue and blood lipids of high-fat diet-fed mice, and to analyze the correlation between iNK T cells and lipid metabolism. Methods Fiftytwo C57 BL/6 mice were selected as the high-fat diet group, and 51 C57 BL/6 mice as the normal control group. The high-fat diet intervention lasted for 12 weeks. At weeks 1, 4, 8, and 12, the epididymal and perirenal fats of mice in both groups were collected and weighed to record the visceral fat mass(VFM), and the changes in body fat content(BFC) were calculated. Flow cytometry and laser scanning confocal microscopy were used to detect the changes of invariant natural killer T(iNK T) cells in visceral adipose tissue. An automatic biochemical analyzer was used to measure the lipid levels in mice, and the correlations of iNKT cells in visceral adipose tissue with VFM, BFC, and serum lipid levels were analyzed. Results At 12 weeks after high-fat diet feeding, the body weight, VFM, BFC, serum total cholesterol(TC), triglyceride(TG) and high-density lipoprotein cholesterol(HDL-C) increased significantly, while the content of invariant natural killer T(iNK T) cells in visceral adipose tissue decreased obviously in the high-fat diet group, as compared with the control group(P<0.01). The iNKT cell number in visceral adipose tissue of mice was negatively correlated with VFM, BFC, serum HDL-C and serum TG(r=-0.293,-0.289,-0.337,-0.199, P<0.05), and was not correlated with serum TC and LDL-C(r=-0.122,-0.082, P>0.05). Conclution VFM is increased and iNK T cell number is decreased in in adipose tissue of high-fat diet-fed mice. The number of iNK T cells is negatively correlated with VFM, BFC, serum HDL-C and TG.更多还原