【摘要】 马铃薯病毒病是制约马铃薯产业健康发展的重要因素之一，建立快速、准确的检测技术是病毒病防控的关键前提。比较分析双抗体夹心酶联免疫吸附测定（Double antibody sandwich-enzyme linked immunosorbent assay,DAS-ELISA）和反转录聚合酶链式反应（Reverse transcription-polymerase chain reaction,RT-PCR）两种方法在检测不同来源马铃薯材料病毒含量差异与适用性，为实现马铃薯产业不同场景样品病毒病的高效快速检测提供依据。采用DAS-ELISA和RT-PCR分别对23份田间显症马铃薯样品和30份微茎尖离体培养材料进行不同病毒[马铃薯Y病毒（Potato virus Y,PVY）、马铃薯S病毒（Potato virus S,PVS）、马铃薯X病毒（Potato virus X,PVX）、马铃薯M病毒（Potato virus M,PVM）和马铃薯卷叶病毒（Potato leafroll virus,PLRV）]检测，对比分析结果表明，大田显症样品，DAS-ELISA与RT-PCR检测结果一致性高；隐症离体培养材料，两次DAS-ELISA检测结果重复性较差，与RT-PCR结果的一致性分别为59.33%和80.66%。本研究认为DAS-ELISA方法适用于田间病毒显症样品的高通量初步筛查与鉴定，RT-PCR适用于脱毒苗、基础种薯等病毒隐症关键材料的精准检测。更多还原

【Abstract】 Potato viral diseases are one of the key factors limiting the healthy development of the potato industry.Establishing rapid and accurate detection techniques is an essential prerequisite for controlling these viral diseases.Comparative analysis of double antibody sandwich-enzyme linked immunosorbent assay(DAS-ELISA) and reverse transcription-polymerase chain reaction(RT-PCR) in detecting viral content differences and applicability across potato materials from various sources provides a basis for achieving efficient and rapid detection of viral diseases in samples across different scenarios within the potato industry. DAS-ELISA and RT-PCR were applied to detect various viruses(Potato virus Y, Potato virus S, Potato virus X, Potato virus M, and Potato leafroll virus) in 23 field symptomatic potato samples and 30 micro-apical shoot tip in vitro cultures. Comparative analysis revealed that the results of DAS-ELISA and RT-PCR were highly consistent for symptomatic samples from fields; for latent in vitro cultures, DAS-ELISA showed poor repeatability in duplicate tests, with concordance rates of 59.33% and 80.66% against RT-PCR, respectively. This study concludes that DAS-ELISA is suitable for high-throughput preliminary screening and identification of field samples with visible virus symptoms, while RT-PCR is appropriate for precise detection of asymptomatic materials such as plantlets in vitro and foundation seed potatoes.更多还原