【摘要】 为了建立中华常春藤植株高效再生的快繁方法，选取茎尖、茎段为外植体，设置0.1%的升汞灭菌时间，以MS培养基为基本培养基，利用正交实验对茎芽苗诱导与植株再生中大量元素浓度和植物激素用量进行优化。结果表明：选取中华常春藤的茎段为外植体，出芽率高达100%。经过洗洁精预处理后，使用0.1%的升汞消毒6 min,灭菌效果最佳，污染率为1.7%,成活率为98.3%;腋芽诱导的最佳培养基为MS+6-BA 4.0 mg/L+GA₃ 1.5 mg/L+NAA 0.2 mg/L,一次诱导率可达93.3%;继代增殖的最佳培养基为MS+6-BA 2.0 mg/L+KT 0.2 mg/L+NAA 0.2 mg/L,45 d后，增值系数可达7.17;试管苗生根的最佳培养基为1/2MS+NAA 0.2 mg/L+KT 0.2 mg/L,45 d后，生根率可达85.1%;以2∶1混合的腐殖土和黄沙土为移栽基质，并在移栽前使用1.5×10^-6的IBA浸泡试管苗根部10 min,可显著提高成活率，移栽成活率达90.0%。更多还原

【Abstract】 To establish an efficient regeneration system for Hedera nepalensis variety sinensis.The stem tip and segment of Hedera nepalensis variety sinensis were used as explants, 0.1% mercuric ascent sterilization time was set, MS medium was used as the basic medium.The concentration of the macro element and plant hormones in axillary buds induction and plantlet regeneration were optimized by orthogonal design. The results showed that the stem segments of Hedera nepalensis variety sinensis were pretreated with detergent and sterilized with 0.1% mercury for 6 min. The contamination rate was only 1.7% and the survival rate was highest at 98.3%. The optimum medium for axillary bud induction was basal MS medium supplemented with 4.0 mg/L 6-BA, 1.5mg/L GA₃ and 0.2 mg/L NAA, the initial induction rate was up to 93.3%. The optimum medium for multiplication culture was basal MS medium supplemented with 2.0 mg/L 6-BA and 0.2 mg/L KT, the multiplication time was 7.17 after 45 days. The best medium for rooting of shoots was basal 1/2 MS medium supplemented with 0.2 mg/L NAA and 0.2 mg/L KT, in which the rooting rate was 85.1% after 45 d. The plantlet was transplanted in the substrate of humus soil and yellow sand soil mixed with 2:1, and the roots of the plantlet were soaked with 1.5×10^-6 IBA 10 min before transplanting can increase the survival rates, the survival rate of transplanting was 90.0%.更多还原