【摘要】 为探明哈茨木霉菌对8年连作土壤栽培甜瓜连作障碍的影响，利用桶栽方式，以未使用哈茨木霉菌为对照（CK），研究哈茨木霉菌对8年连作土壤栽培甜瓜根际土壤酶活性、养分和枯萎病发生的影响。结果表明：与CK相比，哈茨木霉菌DQ002的使用显著提高了薄皮甜瓜根际土壤蔗糖酶、脱氢酶、碱性磷酸酶活性，调节了不同时期脲酶的活性变化，促进了根际土壤pH和电导率的升高。此外，哈茨木霉菌处理降低了根际土壤养分铵态氮、速效磷的质量比，提高了硝态氮的质量比，并降低了枯萎病的发生率。可见，哈茨木霉菌通过调节薄皮甜瓜根际土壤pH、EC以及土壤酶的活性，影响薄皮甜瓜对铵态氮、硝态氮和速效磷的吸收，降低枯萎病的发病率，从而缓解8年连作土壤连作障碍对薄皮甜瓜的伤害。更多还原

【Abstract】 This study investigates the effect of Trichoderma harzianum on the challenges posed by eight years of continuous cultivation of melon. A pot culture experiment was conducted using a control treatment without Trichoderma harzianum(CK) to examine its influence on enzyme activities, nutrient availability, and the occurence of Fusarium wilt in the rhizosphere soil of melon. The results showed that, compared to the CK treatment, the application of Trichoderma harzianum DQ002 significantly enhanced the activities of sucrase, dehydrogenase and alkaline phosphatase in the rhizosphere soil of oriental melon. It also regulated the variations in urease activity over different growth periods and promoted increases in both pH and electrical conductivity(EC) values in the rhizosphere soil. Furthermore, Trichoderma harzianum treatment decreased the levels of ammonium nitrogen and available phosphorus while increasing nitrate nitrogen content, leading to a reduction in the incidence of Fusarium wilt. Notably, the incidence of fusarium wilt was negatively correlated with the activities of sucrose and phosphatase, as well as with the content of nitrate nitrogen in soil. These findings suggest that Trichoderma harzianum modulates the absorption of ammonium nitrogen, nitrate nitrogen, and available phosphorus by regulating pH, EC, and soil enzyme activities in the rhizosphere of oriental melon, thereby reducing Fusarium wilt occurrence and mitigating the detrimental effects of eight years of continuous cropping on oriental melon.更多还原