【摘要】 目的 探讨CD36抗体在体内介导血小板吞噬清除的作用机制。方法 将单克隆抗体（mAb）32-106（IgG2a）以2 mg/kg的浓度注射给C57BL/6J雌鼠，并分别在注射前0 h、注射后0.5 h、1 h、2 h、4 h、6 h及24 h检测小鼠体内血小板数量，通过胃蛋白酶消化处理，获得32-106的F（ab’）₂段，并将其注射给C57BL/6J雌鼠，分析其清除CD36（+）血小板的效果，制备其他亚型的32-106，通过体内实验分析其对CD36（+）血小板的清除作用。结果 体内实验证实，mAb 32-106 IgG2a可导致C57BL/6J雌鼠体内血小板数的明显降低（P<0.05），而mAb 32-106的F（ab’）₂段并不引起雌鼠体内血小板的减少。C57BL/6J雌鼠接受2 mg/kg的32-106 IgG1或32-106 IgG3，也发生血小板数量降低，但与32-106 IgG2a相比，IgG1及IgG3亚型32-106引起的小鼠血小板减少不明显。结论 通过体内实验证实，CD36抗体以Fc依赖途径参与体内CD36（+）血小板的清除，且IgG2a亚型的抗体引起的血小板清除效果显著。更多还原

【Abstract】 Objective To study the mechanism of platelet clearance caused by anti-CD36 antibodies in vivo.Methods The monoclonal antibody （mAb） 32-106 （IgG2a） was injected into the C57BL/6J female mice at a concentration of 2 mg/kg,and the platelet counts of these mice were detected at 0 h before injection,0.5 h,1 h,2 h,4 h,6 h and 24 h after injection.The F （ab’） 2 segment of 32-106 was obtained by digestion with pepsin and then injected into the C57BL/6J mice to analyze its clearance effect on CD36 （+） platelets.Subsequently,other subclasses of mAb 32-106 were generated and injected into the mice to analyze its clearance effect on CD36 （+） platelets.Results mAb 32-106 IgG2a significantly decreased the number of CD36 （+） platelets in C57BL/6J female mice （P<0.05） in vivo.However,the F （ab’） ₂ segment of mAb 32-106 did not cause platelet clearance in female mice.The C57BL/6J female mice receiving 2 mg/kg of 32-106 IgG1 or 32-106 IgG3 also experienced decreased platelet counts.Compared with 32-106 IgG2a,the thrombocytopenia induced by 32-106 IgG3 or 32-106 IgG1 was not significant in mice.Conclusion The clearance of CD36 （+） platelets caused by anti-CD36 antibodies in vivo is in an Fc-dependent pathway,and anti-CD36 IgG2a antibody has a significant effect on CD36 （+） platelet clearance.更多还原