【摘要】 土壤细菌多样性对林地土壤生态功能和多重效益具有至关重要的作用，但不同林龄红松人工林土壤细菌多样性鲜见报道。采用16S rDNA高通量测序技术分析辽东山区不同林龄红松人工林林下表土层和亚土层细菌多样性及群落结构多样性特征。结果表明：红松人工林土壤细菌群主要优势菌门有酸杆菌门、变形菌门、疣微菌门、放线菌门，土壤细菌种群丰富度表现为幼龄林（未成林）>近熟林>中龄林>成熟林>幼龄林。中龄林和近熟林的土壤细菌群落组成最相似。不同林龄红松人工林土壤细菌群落结构和多样性具有显著性差异；除成熟林外，亚表层土壤细菌群落多样性高于表层土壤；幼龄林（未成林）固氮作用功能丰度最高。因此，注重不同林龄红松人工林土壤的维护，对今后维持生产力有重要促进作用。更多还原

【Abstract】 Soil bacterial diversity plays a crucial role in the ecological functions and multiple benefits of forest soil. However, there are few reports on soil bacterial diversity in Pinus koraiensis plantations of different stand ages. In this study, 16S rDNA high-throughput sequencing was used to analyze the diversity and community structure characteristics of soil bacteria in the surface and subsurface layers of Pinus koraiensis plantations of different stand ages in the mountainous region of eastern Liaoning. The results showed that the dominant bacterial phyla in the soil of Pinus koraiensis plantations included Acidobacteria, Proteobacteria, Verrucomicrobia, and Actinobacteria. Soil bacterial richness followed the order: young forest(unestablished) > near-mature forest > middle-aged forest > mature forest > young forest. The soil bacterial community composition was most similar in middle-aged and near-mature forests. Significant differences were observed in the structure and diversity of soil bacterial communities across different stand ages. Except in mature forests, the diversity of soil bacterial communities was higher in the subsurface layer than in the surface layer. The functional abundance of nitrogen fixation was highest in young forests(unestablished). Therefore, paying attention to soil maintenance in Pinus koraiensis plantations of different stand ages will significantly contribute to sustaining future productivity.更多还原