【摘要】 目的 探讨环状RNA hsa＿circ＿0001766在胃癌患者组织和血清中的表达水平及临床应用价值。方法 以正常胃黏膜细胞系GES-1为模板，通过PCR扩增产物后使用Sanger测序分析hsa＿circ＿0001766环化位点。使用核糖核酸酶R（RNase R）处理GES-1 RNA后，应用实时荧光定量PCR（qRT-PCR）检测hsa＿circ＿0001766在RNase R处理组和未处理组的表达水平。采用qRT-PCR检测73例胃癌患者胃癌组织和癌旁组织以及其中34例胃癌患者血清hsa＿circ＿0001766的表达水平。采用ROC曲线评估hsa＿circ＿0001766对于胃癌的临床筛查价值，并分析hsa＿circ＿0001766与胃癌患者临床病理参数的相关性。结果 hsa＿circ＿0001766存在环化位点，RNase R对GES-1细胞中hsa＿circ＿0001766的表达水平无明显影响（t=1.678,P=0.169）。与癌旁组织相比，胃癌组织hsa＿circ＿0001766的表达水平明显上调（U=1 360,P<0.001）;与体检健康者相比，胃癌患者血清hsa＿circ＿0001766的表达水平明显上调（U=375,P<0.001）。胃癌组织hsa＿circ＿0001766筛查胃癌的曲线下面积(AUC^ROC)为0.759（95%CI:0.682～0.837,P<0.000 1）,敏感性为79.45%、特异性为68.49%;血清hsa＿circ＿0001766筛查胃癌的AUC^ROC为0.773（95%CI:0.662～0.884,P<0.000 1）,敏感性为73.53%、特异性为72.12%。胃癌组织hsa＿circ＿0001766的表达水平与淋巴结转移（χ²=5.509,P=0.019）和TNM分期（χ²=5.161,P=0.023）有关。胃癌组织hsa＿circ＿0001766高表达患者的3年生存率显著低于低表达患者（χ²=3.700,P=0.037）。结论 胃癌患者胃癌组织和血清hsa＿circ＿0001766呈高表达，其水平变化对胃癌的筛查及预后评估具有重要意义。更多还原

【Abstract】 Objective To investigate the expression level and clinical application value of circular RNA hsa＿circ＿0001766 in gastric cancer（GC） tissues and serum of patients with GC. Methods The PCR amplification products from GES-1 cells of normal gastric mucosa were analyzed for the cyclic sites of hsa＿circ＿0001766 by the Sanger sequencing technique. The expression levels of hsa＿circ＿0001766 in GES-1 cells treated or untreated with RNase R（RNase R） were detected by real-time fluorescence quantitative PCR（qRT-PCR）. The expression levels of hsa＿circ＿0001766 in GC and adjacent tissues of 73 GC patients and serum samples of 34 GC patients were also detected by qRT-PCR. The receiver operating characteristics（ROC） curve was used to evaluate the clinical diagnostic value of hsa＿circ＿0001766 in GC. The correlations between hsa＿circ＿0001766 and clinicopathological parameters in GC patients were also analyzed. Results There was cyclic sites in hsa＿circ＿0001766 and RNase R had no significant impact on the expression level of hsa＿circ＿0001766 in GES-1 cells（t=1.678, P=0.169）. Compared with adjacent tissues, the expression levels of hsa＿circ＿0001766 in GC tissues were significantly up-regulated（U=1 360, P<0.001）. Compared with healthy controls, the expression levels of hsa＿circ＿0001766 in serum of GC patients were significantly up-regulated（U=375, P<0.001）. The area under the ROC curve(AUC^ROC), sensitivity, and specificity of hsa＿circ＿0001766 in GC tissues for diagnosing GC were 0.759（95%CI: 0.682-0.837, P<0.000 1）, 79.45%, and 68.49%, respectively. The AUC^ROC, sensitivity, and specificity of serum hsa＿circ＿0001766 in diagnosing GC were 0.773（95%CI: 0.662-0.884, P<0.000 1）, 73.53%, and 72.12%, respectively. The expression levels of hsa＿circ＿0001766 in GC tissues were correlated with lymph node metastasis（χ²=5.509, P=0.019） and TNM staging（χ²=5.161, P=0.023）. The 3-year survival rate of GC patients with high expression of hsa＿circ＿0001766 in GC tissues was significantly lower than that with low expression（χ²=3.700, P=0.037）. Conclusion The hsa＿circ＿0001766 in GC tissues and serum of GC patients is highly expressed, and its change in the expression level is of great significance for the diagnosis and prognostic evaluation of GC patients.更多还原