【摘要】 目的 探究核转运因子2(NUTF2)在头颈部鳞状细胞癌（HNSCC）患者中的表达，并分析其与预后的关系。方法 选取2016年3月至2017年2月邯郸市中心医院HNSCC患者269例，以免疫组织化学染色法检测NUTF2表达水平，比较肿瘤组织与癌旁组织、不同肿瘤分期患者NUTF2表达水平。分析NUTF2表达水平与肿瘤分期的相关性及HNSCC患者生存结局的影响因素。比较不同NUTF2表达水平HNSCC患者的生存情况。通过体外实验观察NUTF2表达水平变化对HNSCC细胞增殖和凋亡的影响。结果 HNSCC患者肿瘤组织NUTF2表达水平高于癌旁组织（P <0.05）;HNSCC患者NUTF2表达水平与肿瘤分期呈正相关（P <0.05）;NUTF2表达水平（HR=3.478,95%CI为1.752～6.906）是HNSCC患者生存结局的影响因素（P <0.05）;NUTF2高表达HNSCC患者生存率低于NUTF2低表达患者（HR=0.486,95%CI为0.298～0.793，χ~2=8.345,P=0.004）。NUTF2表达敲低抑制HNSCC细胞增殖，促进细胞凋亡。结论 NUTF2表达水平在HNSCC患者肿瘤组织内异常升高，对患者生存结局存在直接影响，NUTF2高表达提示患者预后较差。更多还原

【Abstract】 Objective To explore the expression of nuclear transport factor 2(NUTF2) in patients with head and neck squamous cell carcinoma( HNSCC) and analyze its relationship with prognosis. Methods 269HNSCC patients from Handan Central Hospital between March 2016 and February 2017 were selected.Immunohistochemical staining was used to detect NUTF2 expression levels,comparing tumor tissues with adjacent tissues and NUTF2 expression across different tumor stages. The correlation between NUTF2 expression levels and tumor staging,as well as factors influencing HNSCC patient survival outcomes,were analyzed. Survival situations of HNSCC patients with different NUTF2 expression levels were compared. In vitro experiments were conducted to observe the effects of NUTF2 expression level changes on HNSCC cell proliferation and apoptosis. Results NUTF2expression levels in HNSCC tumor tissues were higher than in adjacent tissues(P < 0.05); NUTF2 expression levels were positively correlated with tumor staging( P < 0.05). NUTF2 expression level( HR = 3.478, 95%CI1.752～6.906) was a factor influencing HNSCC patient survival outcomes(P < 0.05); HNSCC patients with high NUTF2 expression had lower survival rates compared to those with low NUTF2 expression(HR = 0.486,95%CI0.298～0.793,χ~2 = 8.345,P = 0.004). Knockdown of NUTF2 expression inhibited HNSCC cell proliferation and promoted cell apoptosis. Conclusion NUTF2 expression levels are abnormally elevated in HNSCC tumor tissues,directly affecting patient survival outcomes,with high NUTF2 expression indicating a poorer prognosis.更多还原