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基于DNA条形码和幼期形态学鉴定北虫草菌鳞翅目新寄主

Identification of new lepidopteran hosts of Cordyceps militaris based on DNA barcoding and larval morphology

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【作者】 崔丽丽耿晓平刘腾腾李伟张西秀江欢欢

【Author】 CUI Li-Li;GENG Xiao-Ping;LIU Teng-Teng;LI Wei;ZHANG Xi-Xiu;JIANG Huan-Huan;College of Life Sciences,Shandong Normal University;Mingguang Temple Forest Farm,Linyi;Pingyi County Forestry Administration;

【通讯作者】 刘腾腾;江欢欢;

【机构】 山东师范大学生命科学学院临沂明光寺林场平邑县林业发展中心

【摘要】 鳞翅目昆虫是北虫草菌Cordyceps militaris的主要寄主。固体或液体培养生产的北虫草有益生物功能成分低于寄主培养的北虫草。家蚕是人工培养北虫草使用的主要寄主,但是由于寄主单一且家蚕不是北虫草的天然寄主,这一定程度上限制了北虫草人工培养的质量。本研究利用DNA条形码技术,结合寄主幼虫的形态学特征,对菌体和寄主物种进行了鉴定,发现北虫草菌的3种舟蛾科新寄主:侧带内斑舟蛾Peridea lativitta(Wileman,1911)、黄二星舟蛾Euhampsonia cristata(Butler,1877)、朝鲜新林舟蛾Neodrymonia(Neodrymonia) coreana Matsumura,1922。明确了初步鉴定被寄生状态下侧带内斑舟蛾和朝鲜新林舟蛾幼虫的部分可用特征,为北虫草资源的进一步开发和利用提供了基础数据。

【Abstract】 The primary hosts of Cordyceps militaris are Lepidoptera. Compared to C. militaris produced from host-based cultivation, the favorable bioactive compound of C. militaris produced by solid or liquid culture methods are often inferior. The primary host employed in C. militaris artificial cultivation is silkworm(Bombyx mori). However, the quality of artificially cultivated C. militaris is rather limited because it only has one host and silkworms are not the natural host of the species. The fungal and host species were identified in this study using DNA barcoding technology, which was combined with the morphological characteristics of the host larvae and pupae. Three new hosts of C. militaris from the family Notodontidae were identified: Peridea lativitta(Wileman, 1911), Euhampsonia cristata(Butler, 1877), and Neodrymonia(Neodrymonia) coreana Matsumura, 1922. The preliminary identification of some available characteristics of the larvae of P. lativitta and N.(N.) coreana in the parasitized state was clarified, offering essential details for the future advancement and application of C. militaris resources.

【基金】 山东省外来入侵物种普查项目(2201)
  • 【文献出处】 环境昆虫学报 ,Journal of Environmental Entomology , 编辑部邮箱 ,2025年06期
  • 【分类号】Q969.42;S567.3
  • 【下载频次】4
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