【摘要】 目的 研究不同克隆号的Ki67抗体在不同肿瘤组织上的表达差异。方法 收集3种肿瘤的45例石蜡组织标本，每种肿瘤组织15例，制备多组织芯片。运用免疫组化技术，选取三种不同克隆号的Ki67:MIB-1、SP6、MXR002，其他条件保持一致，进行免疫组化实验。正常组织对照包括扁桃体、阑尾和脂肪组织。结果Ki67(MIB-1)在乳腺肿瘤、肺癌和子宫颈肿瘤的增殖指数分别为66.7%(10/15)、80%(12/15)和86.7%(13/15)，染色强度为弱到中等染色；Ki67(SP6)在乳腺肿瘤、肺癌和宫颈肿瘤的增殖指数为60%(9/15)、73.3%(11/15)和73.3%(11/15)，染色程度为轻至中等；在乳腺癌、肺癌和宫颈癌中，Ki67(MXR002)的增殖指数均达到100%(15/15)，染色强度为强染色，高于Ki67(MIB-1)和Ki67(SP6)(P<0.05)。在常规扁桃体组织和阑尾组织中，Ki67(MXR002)的染色程度及增殖指数也高于Ki67(MIB-1)和Ki67(SP6)(P<0.05)。在脂肪组织样本中，Ki67(MIB-1)抗体出现非特异性染色，而Ki67(MXR002)和Ki67(SP6)抗体则未表现出此类现象。结论 Ki67(MIB-1)和Ki67(SP6)的敏感性均较低，其中Ki67(MIB-1)在脂肪细胞上呈现非特异性染色。因此，推荐采用Ki67(MXR002)进行免疫组化染色。更多还原

【Abstract】 Objective To investigate the expression differences of Ki67 antibodies with different clone numbers in various tumor tissues. Methods A total of 45 paraffin-embedded tissue specimens from three types of tumors were collected, with 15 specimens from each tumor type. A multi-tissue array was prepared. Immunohistochemical staining was performed using three different clones of Ki67: MIB-1, SP6, and MXR002, with all other conditions held constant. Normal tissue controls included tonsil, appendix, and adipose tissue. Results The proliferation indices of Ki67(MIB-1) in breast cancer, lung cancer, and cervical cancer were 66.7%(10/15), 80%(12/15), and 86.7%(13/15), respectively, with weak to moderate staining intensity. The proliferation indices of Ki67(SP6) in breast cancer, lung cancer, and cervical cancer were 60%(9/15), 73.3%(11/15), and 73.3%(11/15),respectively, with light to moderate staining intensity. In breast cancer, lung cancer, and cervical cancer, Ki67(MXR002) showed a proliferation index of 100%(15/15), with strong staining intensity, which was significantly higher than that of Ki67(MIB-1) and Ki67(SP6)(P<0.05). In normal tonsil and appendix tissues, Ki67(MXR002)also exhibited higher staining intensity and proliferation index compared to Ki67(MIB-1) and Ki67(SP6)(P<0.05).In adipose tissue samples, Ki67(MIB-1) antibody exhibited nonspecific staining, while Ki67(MXR002) and Ki67(SP6) antibodies did not show this phenomenon. Conclusion Ki67(MIB-1) and Ki67(SP6) have lower sensitivity, with Ki67(MIB-1) showing nonspecific staining in adipocytes. Therefore, Ki67(MXR002) is recommended for immunohistochemical staining.更多还原