【摘要】 【目的】旨在探讨亚硒酸钠添加方式和添加量对富硒蚯蚓生产的影响，为富硒蚯蚓生产提供参考依据。【方法】在大棚环境条件下，采用质量配比16∶4∶1的3种物料（牛粪、秸秆、沼泥），以亚硒酸钠为硒源，赤子爱胜属大平二号蚯蚓为供试对象，研究了亚硒酸钠添加量（以Se计，硒质量分数分别为80,100,120 mg/kg）及其添加方式（一次性添加，用S表示；分3次添加，用SS表示）对蚯蚓生长、繁殖及其硒含量的影响，以筛选最佳富硒蚯蚓养殖方式。【结果】养殖60 d后，与S处理组相比，SS处理组蚯蚓的存活率、生物量增长率、产茧率和硒富集系数分别提高了8.33%～18.33%、28.85%～60.89%、8.33～131.67个/百条及0.78～0.95。SS处理组蚯蚓硒富集量略低于S处理组，不过蚯蚓体硒含量能维持在231.28～264.30 mg/kg，且以处理SS120为最高。相同添加方式下，过高的外源硒质量分数抑制了蚯蚓的存活率、生物量和产茧率，而SS处理组能保证蚯蚓生长繁殖，同时还能维持较高的硒富集量。【结论】处理SS100为最佳富硒蚯蚓养殖方式，养殖60 d时，蚯蚓的存活率、硒富集量、生物量增长率、产茧率和硒富集系数分别为81.67%、236.70 mg/kg、65.83%、108.33个/百条和2.31。更多还原

【Abstract】 [Objective]This study aims to investigate the effects of sodium selenite addition methods and dosages on the production of selenium-enriched earthworms,thus providing a reference for selenium-enriched earthworm cultivation.[Method]Under greenhouse conditions,three materials(cow manure,straw,and biogas slurry)were mixed in a weight ratio of 16∶4∶1. The effects of sodium selenite addition(with selenium concentrations of 80,100,and 120 mg/kg,calculated as Se)and its supplementation methods(one-time addition,denoted as S;divided into three additions,denoted as SS)on the growth,reproduction,and selenium content of Eisenia fetida(Daping No.2 earthworms)were explored to identify the optimal breeding method for selenium-enriched earthworms with sodium selenite as the selenium source.[Result]Results showed that after 60 days,the survival rate of SS-treated group(8.33%-18.33%),biomass growth rate(28.85%-60.89%),cocoon number(8.33-131.67 per 100 earthworms),and bioaccumulation factor of selenium(0.78-0.95)were increased compared with those the S-treated group.Although selenium accumulation was slightly lower in the SS-treated group,body selenium concentrations remained high(231.28-264.30 mg/kg),with SS120 being the highest.Under the same addition method,excessive selenium inhibited growth and reproduction,but the SStreated group balanced growth and enrichment.[Conclusion]The SS100 method has been identified as the optimal selenium-enriched earthworm farming technique. After 60 days of cultivation,the survival rate,selenium accumulation,biomass growth rate,cocoon production,and selenium bioaccumulation factor of SS100earthworms were 81.67%,236.70 mg/kg,65.83%,108.33 cocoons per 100 earthworms,and 2.31,respectively.更多还原