【摘要】 为了探究香樟叶对农田杂草种子萌发的化感效应,提取了香樟叶中的化感物质,采用室内培养方法,考察香樟叶水浸提液对5种典型农田杂草种子萌发的影响。通过LC-MS对水浸提液进行检测,推测浸提液中影响杂草种子萌发的潜在化感物质。结果表明:1)5种杂草种子对香樟叶水浸提液的化感作用具有显著性差异,不同的杂草种子萌发对香樟叶水浸提液化感效应的响应程度存在差异;2)5种农田杂草对香樟化感作用的敏感顺序由强到弱依次为三叶鬼针草>白车轴草>马唐>苏丹草>紫花苜蓿;3)香樟叶水浸提液对三叶鬼针草、苏丹草、紫花苜蓿、马唐4种杂草根系生长的抑制作用强于其发芽率;而对白车轴草的抑制作用则相反;4)芦丁可能是影响杂草种子萌发的主要因素。香樟叶水浸提液对5种常见农田杂草种子的萌发均有明显的抑制作用,在开发绿色环保生物除草剂方面具有较大的应用潜力。更多还原

【Abstract】 In order to investigate the allelopathic effect of Cinnamomum camphora leaves on the germination of weed seeds in farmland,allelochemicals were extracted from the leaves and tested under controlled indoor cultivation conditions.The extract was used to assess its effects on the seed germination of five representative weed species commonly found in agricultural fields.By detecting the water extract using LC-MS,potential allelopathic substan ces affecting weed seed germination in the water extract were speculated.The research results indicate that:1)the five weed species exhibited significantly different responses to the allelopathic effects of the water extract of C. camphora leaves,with varying degrees of sensitivity observed in seed germination across species;2)the sensitivity of the five weed species to allelopathic effects of C. camphora ranked from strongest to weakest as follows:Bidens pilosa,Trifolium repens,Digitaria sanguinalis,Sorghum sudanense and Medicago sativa;3)the water extract of C. camphora leaves has a stronger inhibitory effect on the root growth of four weed species,namely B.pilosa,S.sudanense,M.sativa and D.sanguinalis,than on their germination rate,while the opposite was observed in T.repens;4)rutin was identified as a potential key allelochemical responsible for the inhibitory effects.Overall,the water extract of C. camphora leaves demonstrates a significant inhibitory effect on the germination of five common weed seeds in farmland,suggesting considerable potential for the development as an environmentally friendly botanical herbicide.更多还原