【摘要】 目的：研究推拿对运动性疲劳大鼠肝组织线粒体代谢和细胞凋亡的影响及其机制。方法：选择雄性SD大鼠，分对照组、模型组及推拿组。应用H-E染色、免疫印迹、RT-qPCR检测大鼠肝组织损伤、线粒体代谢相关分子和细胞凋亡相关分子表达。结果：模型组大鼠肝组织线粒体功能显著损伤，细胞凋亡水平明显上调。推拿干预后，大鼠肝组织线粒体功能显著恢复，细胞凋亡水平也呈现缓解趋势。结论：推拿可减轻运动性疲劳大鼠的肝损伤，该效应可能与调控线粒体功能的AMPK/PGC-1α信号通路及细胞凋亡的caspase-3/cytochrome C信号通路相关。更多还原

【Abstract】 Objective: To investigate the effects of Tuina on mitochondrial metabolism and apoptosis in the liver tissue of rats with exercise-induced fatigue, and to explore the underlying molecular mechanisms. Methods: Male Sprague-Dawley rats were selected, divided into the control group, the model group and the Tuina intervention group.Tissue injury, expression of mitochondrial metabolism-related molecules and apoptosis-related molecules in liver tissue were measured by using H-E staining, Western blotting, RT-qPCR. Results: In the model group, significant damage of mitochondrial function occurred in the liver tissue and the level of cell apoptosis markedly up-regulated.After Tuina, the mitochondrial function was significantly restored, and the level of cell apoptosis also showed a trend of alleviation. Conclusion: Tuina can effectively ameliorate exercise-induced hepatic injury in rats. This effect may be mediated by regulating mitochondrial function through the AMPK/PGC-1α signaling pathway and modulating apoptosis via the caspase-3/cytochrome C pathway.更多还原