【摘要】 目的 探讨白细胞介素-9(IL-9)在骨包虫病骨囊性组织浸润性生长中的作用及其分子机制。方法 收集8例骨包虫病患者累及骨囊性组织的石蜡切片，选择8例骨关节炎患者患病膝关节组织石蜡切片作为对照。qPCR测定骨囊性组织和骨关节炎骨组织中IL-9 mRNA表达水平。将RAW264.7细胞分为Undifferentiation组（Group 1）、MCSF组（Group 2）、MCSF+RANKL组（Group 3）、MCSF+RANKL+包虫抗原B组（Group 4）、MCSF+RANKL+IL-9组（Group 5）。qPCR法测定各组细胞中TRAP和cathepsin K mRNA表达水平。Western blot法测定各组细胞中p-p65、p65、活化T-细胞核因子1(NFATc1)、磷酸化-信号转导与转录激活因子3(p-STAT3)、信号转导与转录激活因子3(STAT3)、磷酸化-细胞外调节蛋白激酶1/2(p-ERK1/2)、细胞外调节蛋白激酶1/2(ERK1/2)的表达水平。结果 与骨关节炎骨组织相比，骨囊性组织中IL-9 mRNA表达水平更高（P<0.05）。与Group 1相比，Group 2、Group 3、Group 4、Group 5细胞中TRAP和cathepsin K mRNA表达水平均升高（P<0.05）,p-p65、p-STAT3和p-ERK1/2水平以及NFATc1的表达水平均升高（P<0.05）；与Group 3相比，Group 5上述检测指标均升高（P<0.05）。结论 IL-9在骨囊性组织中表达升高，并通过上调核因子-κB(NF-κB)/STAT3/ERK1/2信号通路增强诱导破骨细胞分化，可能在骨包虫病的骨侵蚀中发挥重要作用。更多还原

【Abstract】 Objective To investigate the role of interleukin-9(IL-9) in the invasive growth of bone cystic tissue with cystic echinococcosis and its molecular mechanisms.Methods Paraffin sections of bone cystic tissues from 8 patients with cystic echinococcosis were collected,and paraffin sections of affected knee joint tissues from 8 patients with osteoarthritis were selected as controls. qPCR was used to detect the expression levels of IL-9 mRNA in bone cystic tissues and osteoarthritis bone tissues. RAW264. 7 cells were divided into the Undifferentiation group(Group 1), the MCSF group(Group 2), the MCSF+RANKL group(Group 3), the MCSF+RANKL+ echinococcosis antigen B group(Group 4), and the MCSF+RANKL+IL-9 group(Group 5). qPCR was used to detect the expression levels of TRAP and cathepsin K mRNA in cells of each group. Western blot was used to detect the expression levels of p-p65,p65, nuclear factor of activated T-cells 1(NFATc1),phosphorylated signal transducer and activator of transcription 3(p-STAT3),signal transducer and activator of transcription 3(STAT3),phosphorylated extracellular signal-regulated kinase 1/2(p-ERK1/2) and extracellular signal-regulated kinase 1/2(ERK1/2) in cells of each group.Results The expression level of IL-9 mRNA was higher in bone cystic tissues than that in osteoarthritis bone tissues(P<0. 05). Compared with Group 1,the expression levels of TRAP and cathepsin K mRNA in Group 2, Group 3, Group 4 and Group 5 were significantly increased(P<0. 05),as well as the levels of p-p65,p-STAT3 and p-ERK1/2,and the expression levels of NFATc1(P<0. 05);Compared with Group 3,the above indexes in Group 5 were all increased(P<0. 05).Conclusion The expression level of IL-9 is up-regulated in bone cystic tissues,and it enhances the induction of osteoclast differentiation by up-regulating the nuclear factor-κB(NF-κB)/STAT3/ERK1/2 signaling pathway, which may play an important role in bone erosion of bone echinococcosis.更多还原