【摘要】 目的 探究胶质母细胞瘤中miR-330-5p对OY-TES-1的靶向调控关系以及miR-330-5p/OY-TES-1轴对胶质母细胞瘤的迁移能力的影响。方法 生物信息学分析胶质母细胞瘤患者中miR-330-5p的表达水平及其对患者预后生存的影响。将胶质母细胞瘤细胞U251分为miR-330-5p minics组、minics-NC组和miR-330-5p+OY-TES-1过表达组（miR-330-5p minics+pcDNA3.1-OY-TES-1）。使用双荧光素酶报告基因实验检测miR-330-5p对OY-TES-1 3’UTR区域活性的影响；使用qRT-PCR检测OY-TES-1mRNA表达；采用Transwell迁移实验检测miR-330-5p/OY-TES-1轴对胶质母细胞瘤细胞迁移能力的影响。结果 胶质母细胞瘤组织中miR-330-5p的表达明显低于非瘤脑组织和低级别胶质瘤组织(P<0.05)。高表达miR-330-5p的胶质母细胞瘤患者生存期显著长于低表达miR-330-5p患者(P<0.05)。过表达miR-330-5p后OY-TES-1 3’UTR区域活性下降(P<0.05)。与minics-NC组相比，miR-330-5p minics组U251和U87MG细胞中OY-TES-1 mRNA表达水平显著降低(P<0.01)。与minics-NC组相比，miR-330-5p minics组及miR-330-5p+OY-TES-1过表达组迁移细胞数显著减少（P<0.05）；与miR-330-5p minics组相比，miR-330-5p+OY-TES-1过表达组迁移细胞数显著增加（P<0.01）。结论 miR-330-5p靶向调控OY-TES-1抑制胶质母细胞瘤的迁移。更多还原

【Abstract】 Objective To explore the targeted regulatory relationship of miR-330-5p on OY-TES-1 in glioblastoma and the effect of miR-330-5p/OY-TES-1 axis on the migration ability of glioblastoma.Methods Bioinformatics analysis was performed to analyze the expression level of miR-330-5p in patients with glioblastoma and its influence on prognosis and survival of patients. The glioblastoma cells U251 were divided into miR-330-5p minics group, minics-NC group, and miR-330-5p+OY-TES-1 overexpression group(miR-330-5p minics+pcDNA3. 1-OY-TES-1). The effect of miR-330-5p on the activity of OY-TES-1 3’UTR region was detected by double luciferase reporter gene experiment. The expression of OY-TES-1 mRNA was detected by qRT-PCR. The effect of miR-330-5p/OY-TES-1 axis on the migration ability of glioblastoma cells was detected by Transwell migration assay.Results The expression of miR-330-5p in glioblastoma tissue was significantly lower than those in non-tumor brain tissue and low-grade glioma tissue(P<0. 05). The survival time of glioblastoma patients with high expression of miR-330-5p was significantly longer than that of patients with low expression of miR-330-5p(P<0. 05). After overexpression of miR-330-5p, the activity of OY-TES-1 3’UTR region was decreased(P<0. 05). Compared with minics-NC group, the expression levels of OY-TES-1 mRNA of U251 and U87MG cells in miR-330-5p minics group were significantly decreased(P<0. 01). Compared with minics-NC group, the numbers of migrating cells in miR-330-5p minics group and miR-330-5p+OY-TES-1 overexpression group were significantly decreased(P<0. 05). Compared with miR-330-5p minics group, the number of migrating cells in miR-330-5p+OY-TES-1 overexpression group was significantly increased(P<0. 01).Conclusion MiR-330-5p targets OY-TES-1 to inhibit the migration of glioblastoma.更多还原