【摘要】 目的:优化白鲜皮3种有效成分的提取工艺,并对其抗氧化活性进行评价。方法:以液料比、乙醇浓度、提取时间、提取次数为单因素,以白鲜碱、梣酮及黄柏酮3种有效成分的含量为考核指标,采用响应面的方法优化提取工艺条件。以DPPH和ABTS自由基的清除能力评价提取物的体外抗氧化活性。结果:最优提取工艺条件为液料比15:1（mL/g）、乙醇浓度70%、提取时间1.5 h、提取次数1次、提取温度80℃,白鲜碱、梣酮、黄柏酮的得率分别为0.025%、0.083%、0.113%;DPPH和ABTS自由基清除的IC₅₀值分别为2.42和0.72 mg/mL。结论:该工艺同时提取3种有效成分,提取物具有良好的抗氧化能力,为白鲜皮的合理开发及后期深入研究提供参考依据。更多还原

【Abstract】 Objective:To optimize the extraction process of three effectiveconstituents from Dictamnus dasycapus Turcz and evaluate their antioxidant activity.Method:Ethanol concentration,liquid-to-soilid ratio,extraction time,and extraction frequency were used as single factors,and the extraction process was studied using response surface methodology,with the content of Dictamnine,Fraxinellone,and Obacunone as evaluation indicators.Evaluating antioxidant activity of extracts in vitro based on the scavenging ability of DPPH and ABTS free radicals.Result:The optimal extraction process conditions were liquid-to-soilid ratio of 15:1（mL/g）,extraction time of 1.5 hours,ethanol concentration of 70%,extraction frequency of 1 time,and extraction temperature of 80℃.The yield of Bai Xian alkaloid,Fen ketone,and Huang Bai ketone were 0.025%,0.083%,and 0.113%;The IC₅₀ values for DPPH and ABTS free radical scavenging were 2.42 and 0.72 mg/mL,respectively.Conclusion:Three effective constituents were extracted simultaneously in this process and extract has good antioxidant activity,providing a reference basis for the rational development and later in-depth research of Dictamnus dasycapus Turcz.更多还原