【摘要】 以自发荧光的多色混合植物花粉颗粒和花蕊为样本,在基本相同的成像条件下,对激光扫描共聚焦显微术(CLSM)和结构光照明光切片显微术(OS-SIM)进行多通道三维成像对比分析。结果表明:OS-SIM通过宽场成像机制实现了成像速度较传统单点扫描CLSM提升1个数量级;OS-SIM采用发光二极管(LED)光源照明降低了光损伤剂量,较传统单点扫描CLSM降低3个数量级;OS-SIM模块化设计使系统成本降低至CLSM的1/2以下。研究为多色荧光活体成像提供了更具成本效益的解决方案,OS-SIM的技术和成本优势使其在细胞动态三维追踪、神经活动监测等应用领域展现出重要的应用前景。更多还原

【Abstract】 Using multicolor autofluorescent plant pollen grains and flower buds as samples, the performance of confocal laser scanning microscopy(CLSM) and optical sectioning structured illumination microscopy(OS-SIM) are compared under similar imaging conditions for multi-channel 3D imaging. Results demonstrate that OS-SIM achieves one order of magnitude faster imaging speed than conventional single-point scanning CLSM due to its wide-field imaging mechanism. Additionally, OS-SIM reduces photodamage by three orders of magnitude by employing LED illumination instead of laser excitation. The modular design of OS-SIM also lowers system costs to less than half that of CLSM. This study provides a more cost-effective solution for multicolor live-cell fluorescence imaging, highlighting technical and economic advantages of OS-SIM for applications such as dynamic 3D cell tracking and neural activity monitoring.更多还原