【摘要】 玉米赤霉烯酮(ZEN)是常见的饲料污染物，易导致母猪流产、妊娠率低，公猪雌性化、性欲低下，然而玉米赤霉烯酮对公猪精液质量的直接影响尚不清楚。本研究在猪精液常温保存稀释液中添加浓度为0、2、10、50 mmol/L的玉米赤霉烯酮，分别检测常温保存48 h和72 h的精子活率、活力、前向活力、质膜顶体完整性、抗氧化酶活力、活性氧(ROS)水平、丙二醛(MDA)含量、ATP水平等指标。结果表明，玉米赤霉烯酮处理可显著降低精子活率及运动参数(P<0.05),50 mmol/L的玉米赤霉烯酮处理72 h可使精子活率降至11.08±0.61%(P<0.01);50 mmol/L的玉米赤霉烯酮也可显著降低精子质膜完整性、顶体完整性、抗氧化能力、抗氧化酶活性，并降低ATP水平及线粒体膜电位(P<0.05);同时，稀释液中添加50 mmol/L的玉米赤霉烯酮会显著提高精子ROS水平及MDA含量(P<0.05)。因此，在猪精液常温保存稀释液中添加玉米赤霉烯酮会降低精子保存质量及抗氧化能力。研究结果可为进一步探明玉米赤霉烯酮的毒性机制及预防、改善玉米赤霉烯酮导致的公猪生育力下降提供参考。更多还原

【Abstract】 Zearalenone(ZEN) is a common feed pollutant that has potential negative impacts on porcine fertility, which may result in miscarriage and low pregnancy rates in sows, and feminization and decreased libido in boars. However, little is known about the direct effects of ZEN on boar semen quality. In this study, 0, 2, 10 or 50 mmol/L ZEN was added to the semen storage solution, and the sperm viability, movement parameters, the integrity of plasma membrane acrosome, the antioxidant enzyme activity, the reactive oxygen species(ROS) level, the malondialdehyde(MDA) content, and the ATP level were measured at 48 h and 72 h after storage at 17 ℃ in each group. The results showed that the addition of ZEN significantly declined the sperm motility and movement parameters(P<0.05), and that the sperm motility in the 50 mmol/L ZEN treatment group decreased to 11.08±0.61% after 72h(P<0.01). The addition of 50 mmol/L ZEN also significantly reduced the integrity of the sperm plasma membrane, the integrity of the acrosome, the antioxidant capacity, the antioxidant enzyme activity, the ATP level, as well as the mitochondrial membrane potential(P<0.05), while significantly enhanced the ROS level and the MDA content(P<0.05). Hence, the addition of ZEN to the semen storage solution could markedly reduce the sperm quality and the antioxidant capacity during storage at 17 ℃. These results lay the theoretical foundation for delving into the mechanisms for ZEN toxicology and also for prevention and alleviation of the ZEN-induced fertility decline in boars.更多还原