【摘要】 目的 探究EB病毒潜伏膜蛋白1（LMP1）和钙网蛋白（CRT）在鼻咽癌组织中的表达及临床意义。方法 利用TIMER数据库和UALCAN数据库分析CRT mRNA在不同类型肿瘤中的表达情况及其表达水平与其临床病理特征的关系。收集60例鼻咽癌组织和30例鼻咽炎黏膜组织，运用免疫组化检测鼻咽癌组织中LMP1与CRT蛋白的表达情况，分析鼻咽癌组织中LMP1、CRT蛋白的表达水平与鼻咽癌临床病理特征的关系。采用Pearson相关性分析鼻咽癌组织中LMP1与CRT蛋白表达的相关性。结果 数据库结果显示CRT在16种恶性肿瘤中呈现异常高表达，在头颈部鳞状细胞癌（HNSCC）中尤为显著（P<0.05）;HNSCC组织中CRT mRNA在41～60岁、肿瘤TNM分期Ⅱ～Ⅲ期、T₃、T₄期呈高表达（P<0.05）。鼻咽癌组织LMP1、CRT表达水平高于鼻咽炎组织（P<0.05）,两者间存在正相关（P<0.05）,CRT表达水平与T分期有关（P<0.05）。结论 LMP1与CRT在鼻咽癌组织中高表达，两者呈正相关；CRT表达水平与T分期密切相关。更多还原

【Abstract】 Aim To explore the expression and clinical significance of EB virus latent membrane protein 1（LMP1） and calreticulin（CRT） in nasopharyngeal carcinoma tissues. Methods The expression of CRT mRNA in different types of tumors, and the relationship between CRT mRN expression level and clinical pathological characteristics of tumor patients were analyzed by using the TIMER and UALCAN databases. Totally 60 cases of nasopharyngeal carcinoma tissue and 30 cases of nasopharyngeal inflammation mucosal tissue were collected. The expression of LMP1 and CRT proteins in nasopharyngeal carcinoma tissue were detected by using immunohistochemistry, and the relationship between the expression levels of LMP1 and CRT proteins in nasopharyngeal carcinoma tissue and the clinical pathological characteristics of nasopharyngeal carcinoma were analyzed. Pearson correlation analysis was used to investigate the correlation between LMP1 and CRT protein expression in nasopharyngeal carcinoma tissues. Results The database results showed that CRT exhibited abnormally high expression in 16 types of malignant tumors, particularly in head and neck squamous cell carcinoma（HNSCC）（P<0.05）. The expression level of CRT mRNA in HNSCC tissue was significantly increased in patients aged 41-60, tumor TNM staging grade Ⅱ-Ⅲ and T₃, T₄ tumors（P<0.05）. The expression levels of LMP1 and CRT in nasopharyngeal carcinoma tissue were higher than those in nasopharyngitis tissue（P<0.05）, and there was a positive correlation between the two（P<0.05）. CRT expression was related to T stage（P<0.05）. Conclusion LMP1 and CRT are highly expressed in nasopharyngeal carcinoma tissues, and the two are positively correlated; The expression level of CRT is closely related to tumor T stage progression.更多还原