【摘要】 本试验旨在通过人工挤取波纹唇鱼(Cheilinusundulatus)♀的卵和鞍带石斑鱼(Epinepheluslanceolatus)♂的精液,并进行人工混合受精,以观察和描述杂交受精卵胚胎的发育过程及其特征。采用光学显微镜观察并记录各个发育阶段的特征。研究发现,在水温为27.5℃±0.5℃的条件下,波纹唇鱼♀×鞍带石斑鱼♂受精卵直径为(574±31)μm;历时26 h 21 min开始孵化出膜;波纹唇鱼♀×鞍带石斑鱼♂与其他硬骨鱼类胚胎发育特征基本相同,胚胎发育过程可分为受精卵阶段、卵裂阶段、囊胚阶段、原肠胚阶段、神经胚阶段、器官形成阶段和孵化出膜阶段,共7个阶段;波纹唇鱼♀×鞍带石斑鱼♂子一代原肠胚的中晚期与神经胚的早中期高度重合,容易混淆无法区分。研究结果表明,波纹唇鱼♀×鞍带石斑鱼♂子一代胚胎发育时长较长,与直径大小相关的发育时长规律出现偏离,属于特例情况;波纹唇鱼♀×鞍带石斑鱼♂子一代胚胎的发育时长长于父本和母本,显示受到杂交的影响而延长;更接近于父本,表明杂交子一代胚胎发育速度受父本影响较大。更多还原

【Abstract】 This experiment aimed to artificially extract eggs from a female humphead wrasse(Cheilinus undulatus)and the sperm from a male giant grouper(Epinephelus lanceolatus), conduct artificial fertilization, and observe as well as describe the development process and characteristics of the hybrid embryos. The characteristics of each developmental stage were observed and recorded using an optical microscope. The study found that at a water temperature of 27.5℃ ± 0.5℃, the diameter of the fertilized eggs was 574 ± 31 μm; hatching took 26 h and 21 min to start. The embryonic developmental characteristics of the hybrid embryos were the same as those of other bony fish species. The embryonic development process was divided into seven stages: fertilized egg, cleavage, blastula, gastrula, neurula,organ formation, and hatching membrane. The mid-late stage of the original intestine embryo overlapped significantly with the early-middle stage of the neural embryo, causing confusion and making it difficult to distinguish. These study results indicate that the developmental time of the hybrid embryos was longer, deviating from the normal developmental time pattern related to diameter and size, which is unique. The developmental time of the hybrid embryos was longer than those of the parent species, indicating an extension influenced by hybridization. It is more similar to that of the male parent, suggesting a marked influence of the male parent on the developmental speed.更多还原