【摘要】 采用紫外-可见吸收光谱法及荧光光谱法测试生理p H值条件下常春藤皂苷元及楤木皂苷G与牛血清白蛋白（BSA）之间的相互作用，比较常春藤皂苷元及楤木皂苷G与BSA的作用关系，以及二者是否导致BSA构象发生改变。结果显示，紫外吸收光谱和荧光光谱都说明常春藤皂苷元及楤木皂苷G均能与BSA发生结合作用，但荧光猝灭率及结合常数都比较小，说明二者与BSA作用程度较弱。同等条件下楤木皂苷G对BSA的荧光猝灭率及结合常数均大于常春藤皂苷元，说明糖苷化后的楤木皂苷G与BSA作用能力强于常春藤皂苷元。二者均不会造成BSA构象改变。实验结果可为三萜皂苷类化合物作为功能性成分的体内转运、吸收与消除等代谢动力学提供参考。更多还原

【Abstract】 UV-Vis absorption spectroscopy and fluorescence spectroscopy were used to test the interaction between hederagenin and Aralia elata saponin G with bovine serum albumin(BSA) under physiological pH conditions. The binding characteristics of both compounds with BSA were compared, along with an assessment of potential BSA conformational changes. The results demonstrated that both UV absorption and fluorescence spectroscopy confirmed binding between the compounds and BSA, however the low fluorescence quenching rates and binding constants indicated weak interactions. Under the same conditions, the fluorescence quenching rate and binding constant of Aralia elata saponin G against BSA are higher than those of hederagenin, indicating that the glycosylated Aralia elata saponin G has a stronger ability to interact with BSA than hederagenin. Neither of them caused conformational change in BSA. The experimental results can provide reference for the metabolic kinetics of triterpenoid saponins as functional components in vivo, including transport, absorption and elimination.更多还原