【摘要】 为建立一种操作简便的从丹参中提取分离丹参酮IIA，且由操作结果可直观地判断药材的质量的方法，以三氯甲烷-正己烷（3∶2）为洗脱剂，流速2 m L/min，转速200 r/min，分离丹参提取液，收集含有丹参酮IIA的流分，采用Supersil ODS(4.6 mm×150 mm,5 m)色谱柱，以乙腈-0.1%磷酸为流动相，梯度洗脱，流速为1.0 m L/min，柱温为30℃，检测波长为270nm，进样量为10μL，测定和计算目标物质分离获得百分率。结果发现在可见光下，丹参提取液离心薄层色谱呈现3条谱带，收集流分，通过薄层色谱确定目标物质主要在红色谱带，经HPLC测定，计算丹参酮IIA的分离获得百分率约为75%。该方法操作简便、快速，目标物质获得率较高。更多还原

【Abstract】 To establish a simple method for extracting and separating tanshinone IIA from Salvia miltiorrhiza that also allows intuitive assessment of raw herb quality based on operational results, a centrifugal thin-layer chromatography(CTLC) separation was employed using a trichloromethanen-hexane(3:2) eluent at a flow rate of 2 mL/min and rotation speed of 200 r/min. Fractions containing tanshinone IIA were collected. The separation recovery rate of the target compound was determined and calculated via HPLC using a Supersil ODS column(4.6 mm×150 mm, 5 μm), with an acetonitrile-0.1% phosphoric acid mobile phase under gradient elution. The HPLC conditions were: flow rate 1.0 m L/min, column temperature 30 °C,detection wavelength 270 nm, and injection volume 10 μL. Three distinct bands were observed on the CTLC plate under visible light. Target component identification via TLC showed tanshinone IIA predominantly resided in the red band. Subsequent HPLC analysis calculated the separation recovery rate of tanshinone IIA to be approximately 75%. This CTLC method is simple, rapid, and yields a high recovery rate of the target compound.更多还原