【摘要】 目的 采用离子色谱法测定两种不同剂型阿糖胞苷制剂中的氨含量。方法 采用Dionex IonPac CS12A阳离子交换柱（250 mm×4 mm）,Dionex IonPac CG12离子色谱保护柱（50 mm×4 mm）;淋洗液为4 mmol·L^-1甲磺酸溶液，流速为1.1 mL·min^-1,电导检测器，Dionex CERS 300（4 mm）抑制器，抑制电流为13 mA,柱温30℃,池温45℃,进样量25μL。结果 0.12～1.20μg·mL^-1氨与峰面积的线性关系良好（r=0.9995）;氨在注射用阿糖胞苷、阿糖胞苷注射液中的平均回收率分别为91.55%、93.34%,RSD分别为1.22%、2.94%（n=6）。结论 所用方法专属性高，准确可靠，可用于阿糖胞苷制剂中氨的含量测定。更多还原

【Abstract】 OBJECTIVE To determine ammonium in two different kinds of Cytarabine preparations by ion chromatography（IC） method.METHODS The IC method was performed on a Dionex IonPac CS12A（250 mm ×4 mm） cation exchange column and guarded by a Dionex IonPac CG12 column, with 4 mmol·L^-1 methanesulfonic acid as eluent, and the flow rate was 1.1 mL·min^-1.The detector was conductivity detector with a suppressor（ Dionex CERS 300,4 mm）,whose suppression current was 13 mA.The temperature of the column and conductivity detector were 30 ℃ and 45 ℃,respectively,and the injection volume was 25 μL.RESULTS The linearity range of ammonium was 0.12-1.20 μg·mL^-1（r=0.9995）.The average recovery of cytarabine for injection and Cytarabine injection was 91.55% and 93.34%,and the RSD volues were 1.22% and 2.94%（n=6）.CONCLUSION The method is specific, accurate and reliable, which is suitable for the determination of ammonium in Cytarabine preparations.更多还原