【摘要】 目的：探讨新乌梅丸对溃疡性结肠炎（ulcerative colitis, UC）小鼠辅助性T细胞17（T helper cell 17,Th17）/调节性T细胞（regulatory T cell, Treg）免疫平衡的调控作用。方法：SPF级雌性小鼠随机分为空白组、模型组、新乌梅丸组及美沙拉秦组，每组6只。除空白组外，其余小鼠自由饮用2.5%葡聚糖硫酸钠盐（dextran sulfate sodium salt, DSS）溶液，连续7 d。UC模型复制成功后，新乌梅丸组(8.085 g·kg^-1)与美沙拉秦组(606 mg·kg^-1)小鼠以相应药物灌胃，模型组小鼠以PBS灌胃，连续7 d。HE染色观察结肠的病理形态并评分，流式细胞术检测肠系膜淋巴结Th17（CD4⁺IL-17⁺）、Treg（CD4⁺FOXP3⁺）细胞含量，RT-qPCR法检测结肠糖酵葡萄糖转运蛋白1（glucose transporter 1,Glut1）、己糖激酶2（hexokinase 2,HK2）、肌肉丙酮酸激酶同工酶2（pyruvate kinase isozyme type M2,PKM2）表达水平。结果：与空白组比较，模型组小鼠体质量增长率和结肠长度减少、脾脏系数增加（P<0.01）。与模型组比较，新乌梅丸组、美沙拉秦组小鼠体质量增长率和结肠长度增加、脾脏系数减少（P<0.05）。HE染色显示，空白组小鼠结肠黏膜完整。模型组小鼠结肠黏膜明显水肿，肠上皮脱落，绒毛消失，伴炎症细胞浸润。新乌梅丸组和美沙拉秦组小鼠腺体结构得到恢复，水肿和炎症细胞浸润减轻。与空白组比较，模型组小鼠结肠上皮损伤、炎症细胞浸润及总评分增加（P<0.01）。与模型组比较，新乌梅丸组小鼠结肠上皮损伤、总评分降低（P<0.05）。流式细胞术显示，与空白组比较，模型组小鼠肠系膜淋巴结Th17含量增加、Treg含量减少、Th17/Treg比例升高（P<0.01）。与模型组比较，新乌梅丸组、美沙拉秦组小鼠肠系膜淋巴结Th17含量减少、Treg含量增加、Th17/Treg比例降低（P<0.01）。RT-qPCR显示，与空白组比较，模型组小鼠结肠Glut1 mRNA、HK2 mRNA和PKM2 mRNA水平升高，经新乌梅丸治疗后降低（P<0.05）。结论：新乌梅丸可促进UC小鼠结肠黏膜修复，其机制可能与抑制糖酵解、调控Th17/Treg免疫平衡有关。更多还原

【Abstract】 Objective: To investigate the regulatory effects of Xin Wumei Pill on the immune balance of T helper cell 17（Th17）/Regulatory T cell（Treg） in mice with ulcerative colitis（UC）.Methods: SPF-grade female mice were randomly divided into blank group, model group, Xin Wumei Pill group and Mesalazine group, with 6 mice in each group.Except for the blank group, all mice received 2.5% dextran sulfate sodium salt（DSS） solution freely for 7 d.After the UC model was successfully replicated, the Xin Wumei Pill group(8.085 g· kg^-1) and Mesalazine group(606 mg ·kg^-1) were gavaged with the corresponding drugs, and the mice in the model group were gavaged with PBS for 7 d.HE staining was performed to observe the regulatory effects of colorectal cancer in the model group and the model group.The pathological morphology of the colon was observed and scored by HE staining, the number of Th17（CD4⁺IL-17⁺） and Treg（CD4⁺FOXP3⁺） cells in mesenteric lymph nodes was detected by flow cytometry, and the number of glucose transporter 1（Glut1）,hexokinase 2（HK2）,and hexokinase 1（HK2） cells in the colon was detected by RT-qPCR.The expression levels of glucose transporter 1（Glut1）,hexokinase 2（HK2） and pyruvate kinase isozyme type M2（PKM2） were measured by RT-qPCR.Results: Compared with the blank group, the growth rate of body mass and length of colon decreased and the spleen coefficient increased in the model group（P<0.01）.Compared with the model group, the growth rate of body mass, colon length and spleen coefficient of mice in the Xin Wumei Pill and Mesalazine groups increased（P<0.01）.HE staining results showed that the colonic mucosa of mice in the model group was obviously edematous, with the intestinal epithelium peeling off, the villi disappearing, and accompanied by the infiltration of inflammatory cells.The glandular structure of the mice in the Xin Wumei Pill and Mesalazine groups was restored, and the edema and inflammatory cell infiltration were reduced.Epithelial damage, inflammatory cell infiltration and total score increased in mice in the model group compared with the blank group（P<0.01）.Epithelial injury and total score were decreased in mice in the Xin Wumei Pill group compared with that of the model group（P<0.05）.Flow cytometry showed that the number of Th17 in mesenteric lymph nodes increased, the number of Treg decreased, and the ratio of Th17/Treg was elevated in mice in the model group compared with the blank group（P<0.01）.Compared with that of the model group, the number of Th17 in mesenteric lymph nodes decreased, the number of Treg increased, and the ratio of Th17/Treg decreased in mice in Xin Wumei Pill group and Mesalazine group（P<0.01）.RT-qPCR results showed that the levels of colonic Glut1 mRNA,HK2 mRNA,and PKM2 mRNA in the mice in the model group were elevated when compared with that of the blank group and were treated by neo-Umei pill decreased（P<0.05）.Conclusion: Xin Wumei Pill can promote the repair of colonic mucosa in UC mice, and its mechanism may be related to the inhibition of glycolysis and the regulation of Th17/Treg immune balance.更多还原