【摘要】 目的：研究一种高效可靠的脱细胞真皮基质的制备方法。方法：以猪皮为实验材料，用磷脂酶A2与脱氧胆酸钠组合配制脱细胞液，用DNase溶液进行洗涤，对猪皮进行脱细胞处理。对所制备脱细胞真皮基质进行DAPI、HE及免疫组化染色鉴定，并对其胶原等成分进行定量检测。结果：所制备基质脱细胞效果良好，胶原等主要成分含量保留较高。结论：用磷脂酶A2与脱氧胆酸钠组合的脱细胞方法用时短，简单高效，符合脱细胞要求，制成的真皮基质结构及成分保留完整，是一种理想的生物医学材料。更多还原

【Abstract】 Objective To investigate an efficient and reliable method for the preparation of acellular dermal matrix.Methods Porcine skin was selected as the experimental material.Decellularization was performed using a decellularization solution composed of phospholipase A2 and sodium deoxycholate,followed by washing with a 3.4M NaCl high-salt solution and DNase solution.The prepared acellular dermal matrix was characterised using DAPI staining,hematoxylin and eosin (H&E) staining,and immunohistochemical analysis.Additionally,the quantitative assessment of collagen and other components was conducted.Results The prepared matrix demonstrated effective decellularization,exhibiting a high retention of major components such as collagen.Conclusion The decellularization method that employs a combination of phospholipase A2 and sodium deoxycholate is characterised by its efficiency,simplicity,and effectiveness.It fulfils the requirements for decellularization and is a time-efficient process.The resulting dermal matrix exhibits structural integrity and biochemical components,rendering it an ideal biomedical material.更多还原