【摘要】 为探究⁶⁰Co-γ射线对文冠果的辐射诱变效应，以6个文冠果优系种子为材料，采用不同剂量（0～750 Gy）的⁶⁰Co-γ射线进行辐射处理，研究辐射对其种子萌发、幼苗生长及生理生化指标的影响。研究结果表明，在一定辐射剂量范围内，⁶⁰Co-γ射线处理对文冠果种子发芽、出苗及生长具有促进作用，随辐射剂量的增加，三个指标均逐渐受到抑制。辐射处理后的幼苗出现叶形、小叶数量、叶色等形态变异，150～450 Gy辐射剂量处理下，变异率较高且变异类型较丰富。随辐射剂量增加，叶片中抗氧化酶活性、丙二醛（MDA）和可溶性蛋白含量呈先上升后下降的趋势，叶绿素含量和可溶性糖含量呈下降趋势。⁶⁰Co-γ射线辐射对文冠果种子具有明显的诱变效应，并初步确定了适宜辐射剂量在301.10～594.96 Gy之间。本研究可为文冠果诱变育种和创制新种质提供技术参考。更多还原

【Abstract】 In order to explore the radiation mutagenesis effects of ⁶⁰Co-γ ray on Xanthoceras sorbifolium, six Xanthoceras sorbifolium superior line seeds were irradiated with different doses（0-750 Gy） of ⁶⁰Co-γ ray. The effects of radiation on seed germination, seedling growth, and physiological and biochemical indexes were investigated. The results showed that within a certain radiation dose range, ⁶⁰Co-γ ray radiation treatment promoted the germination, seedling emergence and growth of Xanthoceras sorbifolium seeds, while these three indexes were gradually declined as the radiation dose increased. After radiation treatment, the seedlings showed morphological variations in traits such as leaf shape, number of leaflets, and leaf color. Compared to the control, the variation rate under 150-450 Gy radiation dose treatment was higher and the variation types were more abundant. The antioxidant enzyme activity, malondialdehyde（MDA） and soluble protein content in the leaves showed a trend of first rising and then falling with increasing radiation dose, while the chlorophyll content and soluble sugar content showed a downward trend. ⁶⁰Co-γ ray radiation demonstrated obvious mutagenic effects on Xanthoceras sorbifolium seeds, with the optimal dose range preliminarily established at 301. 10 and 594. 96 Gy. These results provide a technical reference for mutation breeding and creating new germplasm of Xanthoceras sorbifolium.更多还原