【摘要】 目的 探究miR-130b-3p、纤维蛋白原样蛋白2(FGL2)、高迁移率族蛋白B1(HMGB1)在癫痫患者血清中的表达。方法 选取2020-02—2023-02郑州大学第一附属医院郑东院区收治的106例癫痫患者为研究对象（研究组），按2∶1的比例随机抽取53例在郑州大学第一附属医院郑东院区进行健康体检的志愿者作为对照组，对2组患者的血清miR-130b-3p、FGL2、HMGB1水平进行检测，并根据患者的发作频率进行分组，42例患者发作频率为2个月1次甚至更少，纳入轻度组，64例患者在2个月内发作次数高于1次，纳入中重度组，比较miR-130b-3p、FGL2、HMGB1表达情况。结果 研究组血清miR-130b-3p相对表达水平（3.56±1.03比1.11±0.23）、FGL2水平[（165.48±11.09）×10~3mg/L比（87.66±12.06）×10~3mg/L],HMGB1水平[（371.15±31.25）×10~3ng/L比（281.12±35.98）×10~3ng/L]均高于对照组（P<0.05）。轻度组患者血清miR-130b-3p相对表达水平（3.11±0.98比3.98±1.12）、FGL2水平[（149.26±38.14）×10~3mg/L比（222.16±45.03）×10~3mg/L]、HMGB1水平[（324.56±45.16）×10~3ng/L比（398.71±39.46）×10~3ng/L]均低于中重度组（P<0.05）。结论 在癫痫患者外周血中，miR-130b-3p、FGL2和HMGB1水平显著升高，且随着病情加重而升高，可为癫痫的诊断和治疗提供重要的生物学信息。更多还原

【Abstract】 Objective To investigate the expressions of serum miR-130b-3p,fibrinogen-like protein 2(FGL2),and high mobility group box 1 protein(HMGB1)in the patients with epilepsy.Methods Totally 106epilepsy patients admitted to Zhengdong Hospital of the First Affiliated Hospital of Zhengzhou University between February 2020 and February 2023 were selected as research group. According to 2∶1 ratio,53 healthy volunteers undergoing physical examinations at the same hospital were randomly recruited as control group. Serum levels of miR-130b-3p,FGL2,and HMGB1 were measured in both groups. Patients were subgrouped based on seizure frequency:42 cases with seizures occurring once every two months or less were classified into mild group,while64 cases with more than one seizure within two months formed moderate-severe group. The expression profiles of miR-130b-3p,FGL2,and HMGB1 were compared between both subgroups.Results The relative expression level of serum miR-130b-3p in the research group(3.56±1.03 vs 1.11±0.23),FGL2 levels[(165.48±11.09)×10~3mg/L vs(87.66±12.06)×10~3mg/L],and HMGB1 levels[(371.15±31.25)×10~3ng/L vs(281.12±35.98)×10~3ng/L]were all significantly elevated compared to the control group(P<0.05). Serum miR-130b-3p relative expression(3.11±0.98 vs 3.98±1.12),FGL2 concentrations[(149.26±38.14)×10~3mg/L vs(222.16±45.03)×10~3mg/L],and HMGB1levels [(324.56 ± 45.16)× 10~3ng/L vs(398.71 ± 39.46)× 10~3ng/L] in the mild subgroup were all significantly reduced compared to the moderate-severe subgroup(P<0.05).Conclusion In the peripheral blood of epilepsy patients,miR-130b-3p,FGL2,and HMGB1 levels are markedly up-regulated,and are concentrations positively correlated with disease severity. These findings suggest their potential as novel biomarkers providing critical biological insights for epilepsy diagnosis and therapeutic development.更多还原