【摘要】 目的：借助网络药理学探讨积雪草防治寨卡病毒（ZIKV）感染的活性成分、作用靶点及潜在作用机制。方法：借助中药系统药理学数据库与分析平台（TCMSP）检索、筛选积雪草中的有效成分，并整合PubChem、SwissTargetPrediction和UniProt数据库，对相应活性成分的潜在靶点进行预测。应用GeneCards、CTD和DisGeNET数据库收集ZIKV感染相关的靶点，利用Venny 2.1在线工具获取交集靶点，并借助Cytoscape 3.7.1软件构建“活性成分-作用靶点”网络。应用STRING数据库和Cytoscape 3.7.1软件构建靶点蛋白相互作用（PPI）网络，并利用CytoHubba插件根据MCC、MNC、EPC和Degree 4种算法筛选核心靶点。利用“clusterProfiler”包对交集靶点进行基因本体论（GO）分析和京都基因与基因组百科全书（KEGG）信号通路富集分析，预测其潜在作用机制。结果：共得到3种积雪草活性成分，256个活性成分作用靶点，3 000个ZIKV感染相关靶点，77个共同靶点。关键活性成分包括洋蓟素、谷固醇和木犀草素，核心靶点有丝氨酸/苏氨酸激酶1(AKT1)、上皮生长因子（EGFR）、肿瘤坏死因子（TNF）、非受体酪氨酸激酶（SRC）、胱天蛋白酶3(CASP3)、丝裂原活化蛋白激酶3(MAPK3)和基质金属蛋白酶9(MMP9)。主要的生物学过程包括氧化应激的反应、正向调节蛋白定位和细胞对化学压力的反应等；关键KEGG信号通路包括PI3K-Akt和MAPK信号通路等。结论：研究初步揭示了积雪草可多成分、多靶点、多通路干预ZIKV感染，发挥治疗作用，为后续开展实验研究提供了理论支撑。更多还原

【Abstract】 Objective:To explore the active ingredients, target proteins and mechanisms of action of Centella asiatica in the prevention and treatment of zika virus(ZIKV) infection through network pharmacology.Methods:The effective components of Centella asiatica were retrieved and screened from TCMSP database. The potential target of the corresponding effective components was predicted by integrating data from PubChem, SwissTargetPrediction, and Uniprot databases. The targe-related to ZIKV infection were collected from GeneCards, CTD and DisGeNET databases. The intersection of targets was obtained using Venny 2.1,and a “active ingredient-target” network was constructed using Cytoscape 3.7.1 software. A protein-protein interaction(PPI)network of targets was constructed using the STRING database and Cytoscape 3.7.1 software, and core targets was selected using the CytoHubba plugin based on four algorithms: MCC, MNC, EPC and Degree. The intersection targets were subjected to Gene Ontology(GO) and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment analyses using the “clusterProfiler”package to predict their potential mechanisms of action.Results:3 active components of Centella asiatica, 256 targets of active components, 3 000 targets related to ZIKV infection, and 77 common targets were identified. Key active components included Cynarin(e), sitosterol, and apigenin. Key biological processes included responses to oxidative stress, positive regulation of protein localization, and cellular response to chemical stress. Key KEGG pathways included PI3K-Akt and MAPK signaling pathways.Conclusion:The study preliminarily reveals that Centella asiatica exerts therapeutic effects in the prevention and treatment of ZIKV infection through multi-component, multi-target, and multi-pathway interventions, providing theoretical support for subsequent experimental research.更多还原