【摘要】 为了确定引起新疆维吾尔自治区某肉鸽养殖场3～4周龄肉鸽腹泻直至死亡的病原并了解该病原的生物学特性，试验对采集的病死鸽病变组织进行细菌分离与纯化，通过菌落形态观察、革兰氏染色与镜检、生化鉴定、病原特异性基因PCR检测、血清型PCR检测、细菌16S r DNA基因的PCR扩增与序列分析对分离菌株进行鉴定，并对分离菌株进行药物敏感性试验、耐药基因和毒力基因的PCR检测及小鼠致病性试验。结果表明:从病死鸽肝脏组织中分离并鉴定到1株鼠伤寒沙门菌（命名为Pigeon-ST-Dec14-2023）。鼠伤寒沙门菌Pigeon-ST-Dec14-2023株对头孢噻肟、卡那霉素、四环素敏感，对阿莫西林、恩诺沙星、环丙沙星中介，对苯唑西林、克林霉素、万古霉素、链霉素、罗红霉素、利福平、杆菌肽耐药;携带β-内酰胺类耐药基因Bla_CTX-M-2，多肽类耐药基因bcr，氨基糖苷类耐药基因rpsl、str B和aad B，氯霉素类耐药基因cat A2及菌毛基因fim A，毒力质粒基因spv D、spv C、spv B、spv A、spvR，毒力岛基因sop B、mgtc、mog A、ssel、inv A;小鼠腹腔注射鼠伤寒沙门菌Pigeon-ST-Dec14-2023株12 h后出现与病死鸽相似的临床症状，并于24 h内全部死亡。说明引起该养殖场肉鸽腹泻直至死亡的病原为鼠伤寒沙门菌，该菌株具有多重耐药性，携带多种耐药基因和毒力基因，对小鼠具有致死性。更多还原

【Abstract】 In order to identify the pathogen causing diarrhea and eventually death of 3-4 week old meat pigeons in a meat pigeon farm in Xinjiang Uygur Autonomous region and understand the biological characteristics of the pathogen,the experiment involved isolating and purifying bacteria from lesion tissues collected from dead pigeons. The isolated strains were identified through colony morphology observation,Gram staining and microscopic examination,biochemical identification,pathogen-specific gene PCR detection,serotype PCR detection,and PCR amplification and sequence analysis of the bacterial 16S r DNA gene. Additionally,drug sensitivity tests,PCR detection of drug resistance genes and virulence genes,and mouse pathogenicity tests were conducted on the isolated strains. The results showed that one strain of Salmonella typhimurium（ named Pigeon-ST-Dec14-2023） was isolated and identified from the liver tissue of diseased pigeons. The Salmonella typhimurium strain Pigeon-ST-Dec14-2023 was sensitive to cefotaxime,kanamycin,and tetracycline; intermediately sensitive to amoxicillin,enrofloxacin,and ciprofloxacin; and resistant to oxacillin,clindamycin,vancomycin,streptomycin,roxithromycin,rifampicin,and bacitracin.It carried the β-lactam resistance gene Bla_CTX-M-2,the polypeptide resistance gene bcr,the aminoglycoside resistance genes rpsl,str B,and aadB,the chloramphenicol resistance gene cat A2,the fimbrial gene fim A,the virulence plasmid genes spvD,spvC,spv B,spv A,and spvR,and the pathogenicity island genes sopB,mgtc,mog A,ssel,and invA. Mice intraperitoneally injected with the Salmonella typhimurium strain Pigeon-ST-Dec14-2023 presented clinical symptoms similar to those of diseased pigeons 12 hours later and all died within 24 hours. It indicated that the pathogen causing diarrhea until death in the meat pigeons of this meat pigeon farm was Salmonella typhimurium. This strain had multi-drug resistance,carried multiple drug resistance genes and virulence genes,and was lethal to mice.更多还原