【摘要】 针对东北地区农业生产过程中氮肥利用率低、土壤退化等问题，研究设置生物质炭0、5、15、25 t·ha^-14个施用量处理，分析土壤氮素循环酶活性的变化，探究生物质炭对碱性土壤连作胁迫条件下氮素循环的影响。研究发现，与对照相比，施加生物质炭的处理在连作条件下硝酸还原酶、脲酶和蛋白酶活性分别显著增加了10.01%～15.45%、17.76%～41.68%和32.78%～77.65%，基本达到轮作水平。同时，氮循环相关酶活性还受到土壤理化性质的显著影响。总之，生物质炭的施用缓解了连作导致的土壤退化问题，使土壤肥力达到与轮作相当的水平，且生物质炭施用量达到15 t·ha^-1时效果最佳。更多还原

【Abstract】 Aiming at the problems of low nitrogen utilization rate and soil degradation during agricultural production in Northeast China,4 application rates of biochar(0,5,15,25 t·ha^-1)were studied to analyze the changes of soil nitrogen cycling enzyme activity and explore the effects of biochar on nitrogen cycling in alkaline soil under continuous cropping stress.It was found that the activities of nitrate reductase,urease and protease were significantly increased by 10.01%-15.45%,17.76%-41.68% and 32.78%-77.65%,respectively,under continuous cropping conditions,which basically reached the crop rotation level.At the same time,the activities of nitrogen cycling-related enzymes were also significantly affected by soil physicochemical properties.In conclusion,the application of biochar alleviated the soil degradation caused by continuous cropping and made the soil fertility equal to that of crop rotation,and the best effect was achieved when the application amount of biochar reached 15 t·ha^-1.更多还原