【摘要】 肠衣废水是一种高盐高蛋白废水，在采用厌氧生物处理工艺时高盐胁迫会抑制微生物活性，从而影响有机质降解效果.为了提高肠衣废水的厌氧生物处理效能，首先采用耐盐适应性进化策略构建出能够耐受3.2%盐度的厌氧产甲烷菌群（进化菌群），然后，从进化菌群中筛选得到一株高耐盐蛋白酶产生菌，命名为原玻璃蝇节杆菌（Arthrobacter protophormiae）A1，其能够在8%盐度下生长并分泌蛋白酶.原玻璃蝇节杆菌A1与进化菌群的复配能够解除高盐胁迫下蛋白质水解受抑问题.在处理3.6%盐度的实际肠衣废水时，耐盐复配菌群的蛋白质降解率和有机质去除率分别达到了93.2%和75.8%.此外，复配菌群还具有一定的耐盐冗余度，在肠衣废水处理过程中展现出较好的稳定性.因此，原玻璃蝇节杆菌A1可为强化肠衣废水等高盐高蛋白废水的厌氧生物处理提供有效的菌种资源，能够显著降低处理成本，具有潜在的应用价值.更多还原

【Abstract】 Casing wastewater is a high-salinity effluent with high-protein content. The high-salinity stress can inhibit microbial activity during anaerobic bio-treatment processes, thereby suppressing the degradation of pollutants. To improve the anaerobic treatment performance of casing wastewater, this study firstly employed an adaptive evolution strategy with salt stress to construct anaerobic methanogenic consortia(evolved consortium) capable of tolerating 3.2% salinity. Subsequently, a highly salt-tolerant protease-producing strain was isolated from the evolved consortium, which was designated as Arthrobacter protophormiae A1. The strain demonstrated growth and protease secretion capabilities under 8% salinity. The co-cultivation of Arthrobacter protophormiae A1 with evolved consortium effectively alleviated the inhibition of protein hydrolysis under high-salinity stress. When treating actual casing wastewater with 3.6% salinity, the salt-tolerant combined microbial consortium achieved protein degradation and organic matter removal efficiencies of 93.2%and 75.8%, respectively. Moreover, the combined consortium possessed notable salt-tolerance redundancy, and demonstrated robust stability during casing wastewater treatment. Therefore,Arthrobacter protophormiae A1 provides an effective microbial resource for enhancing the anaerobic treatment of high-salinity and high-protein wastewater such as casing effluent, which can reduce operational cost and shows a good application prospect.更多还原