【摘要】 文章以大黄鱼鱼皮中提取的鱼皮明胶（fish skin gelatin,FSG）为主体材料，通过乳液模板法制备鱼皮明胶-透明质酸（fish skin gelatin-hyaluronic acid,FSG-HA）复合微球，再利用单宁酸（tannic acid,TA）对复合微球混合交联，构建具有高抗氧化及抗菌活性的单宁酸改性鱼皮明胶-透明质酸（FSG-HA/TA）复合微球。采用动态光散射仪、透射电子显微镜（transmission electron microscope,TEM）、激光扫描共聚焦显微镜（confocal laser scanning microscope,CLSM）、傅里叶变换红外光谱仪（Fourier transform infrared spectrometer,FTIR）和紫外-可见分光光度计等表征FSG-HA和FSG-HA/TA的微观形貌和结构，测定其抗氧化及抗菌活性。结果表明：FSG-HA和FSG-HA/TA均表现出球形结构，单宁酸的交联改性降低了FSG-HA的粒径分布，增强了鱼皮明胶与透明质酸之间的氢键作用，使微球结构更紧密且均匀；FSG-HA和FSG-HA/TA对DPPH自由基和ABTS阳离子自由基的清除率具有质量浓度依赖性，清除率最高分别达93.75%和67.63%。此外，改性后FSG-HA/TA对金黄色葡萄球菌和大肠杆菌的抑制效果显著，尤其是对金黄色葡萄球菌的最高抑菌率达95%。因此，FSG-HA/TA具有较强的抗氧化和抗菌性能，作为食品保鲜和生物医药领域的功能性材料具有巨大的应用潜力。更多还原

【Abstract】 Fish skin gelatin(FSG)extracted fromPseudosciaena croceaskin was used as the main material.Fish skin gelatin-hyaluronic acid(FSG-HA)composite microspheres were prepared by the emulsion template method.Tannic acid(TA)was used to cross-link the microspheres to construct TAmodified fish skin gelatin-hyaluronic acid(FSG-HA/TA)composite microspheres with high antioxidant and antibacterial activities.Dynamic light scattering,transmission electron microscope(TEM),confocal laser scanning microscope(CLSM),Fourier transform infrared spectrometer(FTIR)and ultraviolet-visible absorption spectroscopy were used to characterize the microscopic morphology and structure of FSG-HA and FSG-HA/TA,and their antioxidant and antibacterial activities were determined.The results indicated that both FSG-HA and FSG-HA/TA exhibited spherical structures.The cross-linking modification of TA reduced the particle size distribution of FSG-HA microspheres,enhanced the hydrogen bonding between FSG and HA,and made the microsphere structure more compact and uniform.The scavenging rates of FSG-HA and FSG-HA/TA against DPPH radicals and ABTS cationic radicals were concentration-dependent,with the scavenging rates reaching up to 93.75% and 67.63%,respectively.In addition,the modified FSG-HA/TA had significant inhibitory effects on Staphylococcus aureus and Escherichia coli,with the inhibition rate against Staphylococcus aureus reaching up to 95%.In summary,FSG-HA/TA composite microspheres have strong antioxidant and antibacterial properties and exhibit significant potential as functional materials in the fields of food preservation and biomedicine.更多还原