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燕麦β-葡聚糖的理化特性及其对肌原纤维蛋白乳化性质的影响

Physicochemical characterization of oat β-glucan and its effect on emulsifying properties of myofibrillar protein

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【作者】 王启龙王军辉左淑荣刘咏

【Author】 WANG Qilong;WANG Junhui;ZUO Shurong;LIU Yong;School of Food and Biological Engineering, Hefei University of Technology;Engineering Research Center of Bio-process of Ministry of Education, Hefei University of Technology;

【通讯作者】 王军辉;

【机构】 合肥工业大学食品与生物工程学院合肥工业大学农产品生物化工教育部工程研究中心

【摘要】 文章以不同产地的燕麦为原料,采用热水提取法提取燕麦β-葡聚糖,并用傅里叶变换红外光谱(Fourier transform infrared spectroscopy, FTIR)、气相色谱(gas chromatography, GC)、高效凝胶渗透色谱(high performance gel permeation chromatography, HPGPC)和X射线衍射(X-ray diffraction, XRD)等方法对其进行表征;同时制备肌原纤维蛋白(myofibrillar protein, MP)和燕麦β-葡聚糖复合乳液,探究其乳化活性、乳化稳定性及乳液液滴尺寸。结果表明:所提取的燕麦β-葡聚糖均由葡萄糖组成,3种燕麦β-葡聚糖之间没有明显的理化或结构差异;复合乳液的乳化活性和乳化稳定性均明显高于纯MP乳液,且3种燕麦β-葡聚糖均能显著减小MP乳液液滴的尺寸,提高乳液质量。研究结果为MP和燕麦β-葡聚糖食品乳液体系的构建提供了参考。

【Abstract】 In this paper, oat β-glucans were extracted from oats of different origins by hot water and characterized by Fourier transform infrared spectroscopy(FTIR), gas chromatography(GC), high performance gel permeation chromatography(HPGPC), and X-ray diffraction(XRD). A composite emulsion of myofibrillar protein(MP) and oat β-glucan was prepared to investigate its emulsification activity, emulsification stability, and emulsion droplet size. The results showed that all the oat β-glucans consisted of glucose, and there were no significant physicochemical or structural differences among the three oat β-glucans. The emulsification activity and emulsification stability of the composite emulsion were significantly higher than those of pure MP emulsion. All three oat β-glucans could significantly reduce the droplet size of MP emulsion and improve the emulsion quality. The results may provide a reference for constructing MP and oat β-glucan based food emulsion systems.

【基金】 国家自然科学基金资助项目(31771974);中央高校基本科研业务费专项资金资助项目(JZ2020YYPY0246)
  • 【文献出处】 合肥工业大学学报(自然科学版) ,Journal of Hefei University of Technology(Natural Science) , 编辑部邮箱 ,2025年05期
  • 【分类号】TS201.2
  • 【下载频次】95
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