【摘要】 【目的】明确引起福建省闽清县白樟镇溪南村地区水稻细菌性穗枯病的病原菌及其生物学特性,为防治该病害提供有效指导依据。【方法】采用组织分离法对病原菌进行分离纯化,通过柯赫氏法则验证其致病性,结合病原菌的形态学特征、生理生化反应以及分子生物学方法明确病原菌的分类地位。【结果】从采集的水稻病样分离纯化获得7株细菌菌株,经柯赫氏法则证明这7株细菌菌株均是该病害的病原菌。细菌菌株为革兰氏阴性菌;在NA、KB、PDA、CCNT培养基上的菌落形态均为圆形,颜色为淡黄色至土黄色,边缘光滑。该细菌的最适生长温度为34℃,最适生长pH为7.0。供试细菌菌株与对照菌株Burkholderia glumaeGX16的生理生化结果一致;颖壳伯克氏菌特异性引物Bglu3F/R扩增获得了单一的目的条带;结合16S rDNA、基因gyrB和rpoD序列分析,将供试菌株鉴定为颖壳伯克氏菌(B. glumae)。【结论】本研究将引发福建省闽清县白樟镇溪南村水稻细菌性穗枯病的病原菌确定为颖壳伯克氏菌。更多还原

【Abstract】 【Aim】 The pathogen causing bacterial panicle blight of rice in Xinan Village, Baizhang Town, Minqing County, Fujian Province and its biological characteristics are studied, with the aim of providing guidance for the prevention and control of this disease. 【Method】 The tissue culture method was employed to isolate the pathogen, the pathogenicity of which was then verified by Koch′s postulates. The morphological characteristics, physiological and biochemical properties, and molecular evidence were used to identify the pathogen. 【Result】 Seven bacterial strains were isolated from rice samples, which were confirmed by Koch′s postulates to be the pathogens of bacterial panicle blight. The bacterial strains were gram-negative, forming round and light yellow to earth yellow colonies with smooth edges on NA, KB, PDA, and CCNT plates. The strains showed optimum growth at 34 ℃ and pH7.0. The physiological and biochemical test results of the strains were consistent with those of the control strain Burkholderia glumae GX16. A single target band was obtained by amplification of Bglu3F/R, specific primers of B. glumae. Based on the results above and 16S rDNA, gyrB, and rpoD sequences, the strains were identified as B. glumae. 【Conclusion】 The pathogenic bacteria causing bacterial panicle blight of rice in Xinan Village, Baizhang Town, Minqing County, Fujian Province is B. glumae.更多还原