【摘要】 查尔酮合成酶(CHS)是类黄酮合成途径的关键起始酶，参与合成黄酮、黄酮醇、异黄酮、花青素等代谢物，而这些黄酮类代谢物在植物抗逆方面扮演着重要角色。为了研究CHS在燕麦幼苗响应干旱胁迫中的作用，从前期的全长转录组数据中筛选到1个燕麦CHS基因，命名为AsCHS,并对其进行克隆、生物信息学、亚细胞定位和表达模式分析。结果表明，AsCHS基因编码398个氨基酸，蛋白序列包含CHS家族特异标签序列，是疏水的不稳定蛋白，属非跨膜蛋白，定位于细胞核和细胞质。根据二级结构预测，AsCHS主要包含α-螺旋和无规则卷曲。启动子区顺式作用元件预测表明，该基因含有响应干旱胁迫和多个激素信号途径相关的顺式作用元件。系统进化树显示，AsCHS与黑麦草、早熟禾和南极发草的亲缘关系较近。亚细胞定位表明，AsCHS蛋白定位在细胞核和细胞质。与对照组相比，AsCHS在干旱胁迫下燕麦幼苗不同萌发时间的表达模式由波动表达改变为递增表达，由根中表达量最高改变为在叶中表达量最高，且在叶片中的表达存在显著差异。本研究为揭示AsCHS在燕麦响应干旱胁迫的功能奠定了基础。更多还原

【Abstract】 Chalcone synthase(CHS)is the initial and crucial enzyme in the flavonoid biosynthesis pathway, responsible for the synthesizing of metabolites such as flavones, flavonols, isoflavones, and anthocyanins, which play a vital role in enhancing plant stress resistance.In order to explore the role of CHS genes in the drought stress response of oat seedlings, it identified a CHS gene from the full-length transcriptome data of oats, named AsCHS.Gene cloning, bioinformatics analysis, subcellular localization, and expression pattern analysis were conducted.The results showed that the AsCHS gene encoded a protein composed of 398 amino acids and had a CHS family-specific tag sequence.This protein was hydrophobic and unstable.It was a non-transmembrane protein and was located in the nucleus and cytoplasm.Secondary structure prediction showed that AsCHS was mainly composed of α-helices and random coils.The analysis of the cis-acting elements within the promoter region revealed that the gene contained cis-elements associated with drought stress response and multiple hormone signaling pathways.Phylogenetic tree analysis showed that AsCHS was closely related to its counterparts in Lolium perenne,Poa annua,and Deschampsia antarctica.Subcellular localization indicated that the AsCHS protein was localized in the nucleus and cytoplasm.Compared with the control group, the expression pattern of AsCHS in oat seedlings under drought stress changed from fluctuating expression to incremental expression with different germination time, shifting from the highest expression level in roots to the highest in leaves, with significant differences observed in leaves expression.It laid a foundation for elucidating the function of AsCHS in the drought stress response of oats.更多还原