【摘要】 为了探究地胆草抗流行性感冒(以下简称流感)的活性成分，应用液质联用技术对地胆草中化学成分进行鉴定。首先，采用Swiss Target Prediction数据库对分析鉴定得到的化学成分作用靶点进行预测；其次，使用GEO数据库筛选用于构建共表达基因模块的基因数据集，并与预测靶标进行匹配映射，获取抗流感潜在靶点，再利用基因本体论(gene ontology, GO)及京都基因与基因组百科全书(kyoto encyclopedia of genes and gnomes, KEGG)富集分析方法对潜在靶点进行分析；然后，同时构建地胆草成分-抗流感靶点-通路网络拓扑图，解析地胆草抗流感的作用机制；最后，利用分子对接技术筛选与核心靶标相作用的化合物。结果表明：地胆草中共鉴定出48种化学成分，可能作用于607个相关靶点，得到7个共表达基因模块，53个潜在抗流感作用靶点；地胆草中有机酸类、黄酮类、倍半萜内酯类等成分均与核心靶点具有良好的结合性能，其潜在调控通路主要由HIF-1信号通路、TNF信号通路、Toll样受体信号通路组成。预测的靶点结果及地胆草对抗流感的“多成分-多靶点-多途径”作用机制，可为抗流感临床应用提供一定的理论依据。更多还原

【Abstract】 In order to explore the active ingredient of Elephantopus scaber L., the components of Elephantopus scaber L. were identified based on UPLC-Q-TOF-MS/MS. Firstly, The Swiss Target database was used to predict the action targets of the chemical components identified. Secondly, The GEO database was used to screen the gene data set for the construction of co-expression gene modules, and matched with the predicted targets to obtain the potential anti-influenza targets, and the potential targets were analyzed by GO and KEGG enrichment analysis. Then, The composition-anti influenza target-pathway network topology of Elephantopus scaber L. was also constructed to analyze the anti-influenza action mechanism of Elephantopus scaber L.. Finally, the compounds that interact with the potential biomarkers were screened virtually by molecular docking technique. The results show that, a total of 48 components were identified from Elephantopus scaber L., which were analyzed to obtain 607 action targets, 7 co-expressed gene modules, and 53 potential anti-influenza action targets. The organic acids, flavonoids and sesquiterpene lactones have good binding properties to the core targets, and their potential regulatory pathways mainly consisted of HIF-1 signaling pathway, TNF signaling pathway and Toll-like signaling pathway. The predicted targets and “multi-component, multi-target, multi-pathway” mechanism of action of Elephantopus scaber L. anti-influenza can provide certain theoretical basis for the clinical application of treatment of anti-influenza.更多还原