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外源葡萄糖对黄瓜嫁接苗生长及光合特性的影响

Effects of exogenous glucose on the growth and photosynthetic characteristics of grafted cucumber seedlings

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【作者】 毕格; 黄晓东; 申宝营; 焦玉兰; 林碧英; 郑秀玉;

【Author】 BI Ge;HUANG Xiaodong;SHEN Baoying;JIAO Yulan;LIN Biying;ZHENG Xiuyu;College of Horticulture, Fujian Agriculture and Forestry University;Fuzhou Changyu Agricultural Development Co., Ltd.;

【通讯作者】 申宝营;

【机构】 福建农林大学园艺学院; 福州昌育农业开发有限公司;

【摘要】 [目的]探究外源葡萄糖对黄瓜嫁接苗生长发育及光合特性的影响,为其嫁接技术体系的优化提供参考。[方法]以‘壮士’南瓜为砧木,‘中农26号’黄瓜为接穗,采用改良版顶插固定嫁接法进行嫁接。在嫁接愈合期分别喷施13.9、27.8、41.7、55.6 mmol·L-1葡萄糖溶液(T1~T4处理),以喷施清水为对照(CK),测定不同处理下黄瓜嫁接苗的形态指标、叶绿素荧光参数及光合参数。[结果]T2处理最有利于黄瓜嫁接苗的生长,其株高(74.42 mm)、茎粗(5.06 mm)、地上部鲜质量(5.69 g)和干质量(0.554 g)均达到最大。T2处理黄瓜嫁接苗在嫁接愈合期的光合作用最强,其砧穗子叶实际光化学量子效率[Y(Ⅱ)]和光化学淬灭系数(qP)均最大,且与其他处理差异显著,而PSⅡ调节性能量耗散[Y(NPQ)]最小。嫁接后第9天,T2处理黄瓜嫁接苗接穗子叶净光合速率(Pn)、胞间CO2浓度(Ci)、蒸腾速率(Tr)和气孔导度(Gs)均达到最大,且与CK差异显著;嫁接后第6天,T2处理嫁接苗砧木子叶Pn、Tr、Gs均最大,且与其他处理差异显著。[结论]在嫁接愈合期喷施27.8 mmol·L-1葡萄糖溶液(T2处理)可促进黄瓜嫁接苗的生长,增强其光合作用,有利于嫁接苗壮苗的形成。

【Abstract】 [Purpose] To investigate the effects of exogenous glucose on the growth, development, and photophsynthetic characteristics of grafted cucumber seedlings and provide a basis for optimizing the grafting system of cucumber. [Method] A modified version of the top-plugging method was used for grafting with the pumpkin variety ‘Zhuangshi’ as the rootstock and the cucumber variety ‘Zhongnong 26’ as the scion. During the graft healing period, 13.9, 27.8, 41.7, 55.6 mmol·L-1 were sprayed with glucose solutions(T1-T4 treatments), take the spraying of clear water as the control(CK). The morphological indices, chlorophyll fluorescence parameters, and photosynthetic parameters of the grafted cucumber seedlings under different treatments were determined. [Result] T2 was most favourable to the growth of grafted cucumber seedlings, which reached the maximum in plant height(74.42 mm), stem diameter(5.06 mm), aboveground fresh mass(5.69 g), and aboveground dry mass(0.554 g). The grafted cucumber seedlings treated with T2 had the strongest photosynthesis in the graft healing stage, with actual photochemical quantum efficiency [Y(Ⅱ)] and photochemical quenching coefficient(qP) of their rootstock and scion cotyledons being the maximum and significantly different from those of other treatments, while PSⅡ regulatory energy dissipation [Y(NPQ)] was the minimum. On the 9th day after grafting, the net photosynthetic rate(Pn), intercellular carbon dioxide concentration(Ci), transpiration rate(Tr), and stomatal conductance(Gs) of scion cotyledons in grafted cucumber seedlings all reached the maximum in the T2, with significant differences compared with CK. On the 6th day after grafting, the Pn, Tr, and Gs of rootstock cotyledons in grafted seedlings were the maximum in T2, and significantly different from those of other treatments. [Conclusion] Spraying 27.8 mmol·L-1 glucose solution(T2) during the grafting healing stage promoted the growth and enhanced the photosynthesis of grafted cucumber seedlings, being favourable to the formation of strong grafted cucumber seedlings.

【基金】 福建省教育厅中青年教师教育科研项目“光照调控黄瓜嫁接苗愈合进程机理研究”(JAT210076);福建农林大学乡村振兴服务团队“设施种苗工程服务团队”(11899170126);福建农林大学园艺学院青年学术骨干培养基金
  • 【文献出处】 亚热带农业研究 ,Subtropical Agriculture Research , 编辑部邮箱 ,2025年02期
  • 【分类号】S642.2
  • 【下载频次】15
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