【摘要】 急尖长苞冷杉（Abies georgei var. smithii）在我国的保护级别为二级，对环境有较高的敏感度，目前该种群有减少的趋势，种子繁殖是其天然更新的主要方式，因此，其种子生活力对该种群的延续至关重要。为了更加科学、高效地检测其种子生活力，本研究采用TTC法检测种子生活力，通过正交试验设计筛选出浸种温度、浸种时间、TTC浓度、染色温度、染色时间5个因素的最优组合。结果表明：（1）TTC法检测急尖长苞冷杉种子的生活力具有可行性，相比发芽试验，其在种子质量判断上更能节约时间成本；（2）各因素对急尖长苞冷杉种子生活力检测的影响由大到小依次为：TTC浓度>浸种温度>染色温度>浸种时间>染色时间；（3）TTC法检测急尖长苞冷杉种子生活力的最适条件为：种子在20℃浸泡12 h后，用0.3%TTC的溶液在30℃黑暗中染色12 h。更多还原

【Abstract】 The protection level of Abies georgei var. smithii acuminata in China is Grade II., which is highly sensitive to the environment. At present, the population of Abies georgei var. smithii has a decreasing trend, and seed reproduction is the main way of its natural regeneration. Therefore, its seed viability is crucial to the continuation of the population. In order to detect seed viability more scientifically and efficiently, the TTC method was used to detect seed viability, and the optimal combination of 5 factors including soaking temperature, soaking time, TTC concentration, dyeing temperature and dyeing time was selected through orthogonal experimental design. The results showed that:(1) The TTC method was feasible to detect the viability of Abies georgei var. smithii seeds. Compared with the germination test, the TTC method could save more time and cost in seed quality judgment;(2) The effects of various factors on the viability of Abies georgei var. smithii seeds were as follows: TTC concentration > soaking temperature > dyeing temperature > soaking time > dyeing time;(3) The optimum conditions for the determination of seed viability by TTC were as follows: after soaking seeds at 20 ℃ for 12 h, the dye was stained with 0.3% TTC solution at 30 ℃ for 12 h in the dark.更多还原