【摘要】 为了研究油脂在塑料制造中的潜在应用价值，以米根霉(Rhizopus oryzae)CGMCC 3.5040全细胞为催化剂，采用化学-酶法生产环氧油脂。本文考察了不同溶剂处理细胞对酶活的影响；研究了不同酶量下油脂及其衍生物环氧化的可行性；并对溶剂体系、温度、过氧化氢用量以及反应时间进行优化以提高油酸甲酯环氧化效率；最后，对比了不同增塑剂配方对应的材料力学性能。结果证明，经丙酮处理后的米根霉全细胞，其脂肪酶活力高达395.48 U/g。在丙酮处理细胞的基础上，对油酸甲酯环氧化反应条件进行了优化，在米根霉用量为200 U/g、过氧化氢与油酸甲酯的物质的量比为2∶1、反应温度为35℃时，无溶剂体系中油酸甲酯反应16 h后，取得了最佳效率，转化率达到94.69%,环氧值为5.24%。此外，PVC-MEM-60的共混物拉伸强度为10.28 MPa,断裂伸长率为300.54%,弹性模量为51.82 MPa。更多还原

【Abstract】 In order to study the potential application value of oil in plastic manufacturing, the whole cell of Rhizopus oryzae CGMCC 3.5040 was used as catalyst to produce epoxy oil by chemical-enzymatic method. In this paper, the effects of different solvents on enzyme activity were investigated. The feasibility of epoxidation of oil and its derivatives under different enzyme amounts was studied. The solvent system, temperature, hydrogen peroxide dosage and reaction time were optimized to improve the epoxidation efficiency of methyl oleate. The mechanical properties of materials corresponding to different plasticizer formulations were compared. The results showed that the lipase activity of Rhizopus oryzae treated with acetone was as high as 395.48 U/g. On the basis of treating cells with acetone, the reaction conditions of epoxidation of methyl oleate were optimized. Under the conditions of 200 U/g Rhizopus oryzae dosage, 2∶1 molar ratio of hydrogen peroxide to methyl oleate, and reaction temperature of 35℃, the best efficiency was obtained after reacting methyl oleate for 16 h in a solvent-free system, with a conversion rate of 94.69% and an epoxy value of 5.24%. In addition, the tensile strength, elongation at break and elastic modulus of PVC-MEM-60 blends were 10.28 MPa, 300.54% and 51.82 MPa, respectively.更多还原