【摘要】 为探究不同光质对胀果甘草Glycyrrhiza inflata_Batal.播种苗生理特性和活性成分含量的影响,本研究对盆栽胀果甘草进行红光（R）、蓝光（B）和红蓝光配比（4R1B、3R1B、2R1B、1R1B）处理,并以白光（W）处理为对照（CK）,考察不同处理对胀果甘草生长、生理指标及甘草苷、甘草酸含量的影响。结果表明,红光和红蓝光配比处理下胀果甘草的芦头直径、地上部干重均大于蓝光处理,其中4R1B处理显著高于CK与其他处理（P<0.05）;红蓝光配比处理下胀果甘草的根冠比均大于蓝光和红光处理。与CK相比,红光处理和蓝光处理下叶片的净光合速率（P_n）和气孔导度（G_s）较低,而部分红蓝光配比处理下叶片的P_n大于CK及其他处理;红光和红蓝光配比处理下叶片的叶绿素a（Chl a）含量和叶绿素b（Chl b）含量均高于蓝光处理。红光和部分红蓝光配比处理下叶片的超氧化物歧化酶（SOD）活性高于蓝光处理,而红光和所有红蓝光配比处理下丙二醛（MDA）含量均低于CK和蓝光处理。脯氨酸含量高低为蓝光处理>红蓝光处理>红光处理;蓝光和红蓝光配比处理下可溶性糖含量较低;红光、蓝光、3R1B、2R1B处理下可溶性蛋白含量高于CK。红光及红蓝光配比（除3R1B外）处理下甘草苷含量均高于蓝光处理,而红光和4R1B处理下甘草苷、甘草酸的含量均高于CK和蓝光处理,其中4R1B处理下两种成分含量最高。综上,蓝光抑制光合效率并引发高强度胁迫,限制了生物量积累,而红光和红蓝光配比通过协同优化光合碳同化与胁迫响应平衡,促进胀果甘草播种苗的生长发育以及活性成分积累,尤其4R1B处理促进生长和活性成分积累的效果明显。更多还原

【Abstract】 To investigate the effects of different light qualities on the physiological characteristics and active ingredient content of seedlings of Glycyrrhiza inflata Batal.,potted G.inflata seedlings were treated with red light(R),blue light(B) and red-blue light combinations(4R1B,3R1B,2R1B,1R1B),with white light(W) treatment as the control(CK).The effects of these treatments on the growth,physiological indices,and content of liquiritin and glycyrrhizic acid were analyzed.The results showed that the rhizome diameter and shoot dry weight of seedlings under red light and red-blue light were greater than those under blue light.The 4R1B group had higher rhizome diameter and shoot dry weight than CK and other treatments(P<0.05).Red-blue light combinations showed higher root-to-shoot ratios than red or blue light treatment.Compared with CK,red light and blue light treatments exhibited decreases in net photosynthetic rate(P_n) and stomatal conductance(G_s),while some red-blue light combinations showed increases in P_n.Under red light and redblue light combinations,the content of chlorophyll a(Chl a) and chlorophyll b(Chl b) in leaves was higher than that under the blue light treatment.The Super Oxide Dismutase(SOD) activity under red light and some red-blue light treatments was higher than that under blue light treatment,whereas the malondialdehyde(MDA) content under red and all red-blue light treatments was lower than that under CK and blue light.The proline content followed the trend of blue light>red-blue light>red light.The soluble sugar content under blue light and red-blue light treatments was higher than under CK.The soluble protein content under red light,blue light,3R1B,and 2R1B was higher than that under CK.Red and red-blue light(except 3R1B) treatments significantly increased liquiritin content compared with the blue light treatment.Red light and 4R1B treatments had higher content of liquiritin and glycyrrhizic acid than CK and blue light treatment.Notably,the 4R1B treatment achieved the highest levels of both liquiritin and glycyrrhizic acid.In conclusion,blue light inhibits photosynthetic efficiency and induces high stress,which limits biomass accumulation.Red-blue light combinations optimize the balance between photosynthetic carbon assimilation and stress response,promoting the growth and development as well as the active ingredient accumulation of G.inflata seedlings.Particularly,the 4R1B treatment demonstrates the best performance.更多还原