【摘要】 为探究槲蕨多糖（Drynaria roosii Polysaccharide,DRP）对脓毒症小鼠免疫功能的影响，本研究通过建立盲肠结扎穿孔（CLP）诱导的小鼠脓毒症模型，探究DRP的抗炎作用及其对小鼠肺、肠道和脾脏组织损伤的保护效应。结果 表明：DRP主要由5种单糖组成，分别为甘露糖（5.41%）、葡萄糖（32.41%）、阿拉伯糖（6.85%）、半乳糖（18.25%）和鼠李糖（21.17%），其平均分子量为197.15kDa；与对照（Control）组相比，CLP组小鼠72h存活率明显降低，DRP+CLP组小鼠72h存活率明显升高；与CLP组相比，DRP+CLP组中HMGB1/TLR4/NF-κB信号通路相关蛋白（HMGB1、TLR4、NF-κB、MyD88、IKKα和IKKβ）及促炎细胞因子基因（IL-1β、IL-6、IL-17、IL-23、TNF-α）表达均显著降低（P<0.05），但抗炎细胞因子IL-10基因的表达显著升高（P<0.05）。本研究结果显示，DRP可通过调控HMGB1/TLR4/NF-κB信号通路，有效抑制脓毒症引发的促炎细胞因子过度释放，从而减轻全身炎症反应。后续可在动物实验结果的基础上进行细胞层面的探究，以更加全面地阐述DRP对小鼠脓毒症的缓解作用机制。更多还原

【Abstract】 This study aims to explore the effect of Drynaria roosii Polysaccharide(DRP)on the immune function in sepsis mice.The mouse model of sepsis was established by Cecal Ligation and Puncture(CLP)to investigate the anti-inflammatory effect and the protective effect on the lung,intestine,and spleen of DRP.The results showed that DRP was mainly composed of five monosaccharides:mannose(5.41%),glucose(32.41%),arabinose(6.85%),galactose(18.25%),and rhamnose(21.17%).The average molecular weight of DRP was determined as 197.15 kDa.Compared with the Control group,the 72 hsurvival rate of mice significantly decreased in the CLP group,while the decrease was attenuated in the DRP+CLP group.Compared with the CLP group,the DRP+CLP group showed down-regulated expression levels of HMGB1/TLR4/NF-κB signaling pathway-associated proteins(HMGB1,TLR4,NF-κB,MyD88,IKKα,and IKKβ),down-regulated mRNA levels of pro-inflammatory cytokines(IL-1β,IL-6,IL-17,IL-23,and TNF-α)(P<0.05),and an up-regulated mRNA level of the anti-inflammatory cytokine IL-10(P<0.05).The results indicated that DRP inhibited the sepsis-induced excessive release of pro-inflammatory cytokines by regulating the HMGB1/TLR4/NF-κB signaling pathway,thereby reducing the systemic inflammatory response.On the basis of the results of animal experiments,cell-level exploration can be carried out to comprehensively explain the mechanism underpinning the alleviating effect of DRP on sepsis in mice.更多还原