【摘要】 过氧乙酸（PAA）用于消毒能有效灭活细菌并控制消毒副产物（DBPs）生成，但存在细菌重新复活的问题。为此，探究了紫外/过氧乙酸（UV/PAA）工艺对铜绿假单胞菌的灭活性能和机理。通过灭活、暗修复、膜渗透性分析和电镜扫描等实验及表征方法，发现UV/PAA工艺的灭活性能优于单独紫外线或过氧乙酸，这是因为在UV/PAA体系中产生了自由基和细胞内活性氧（ROS）。铜绿假单胞菌的膜通透性随着暴露在UV/PAA体系中时间的增加而增加，且细胞表面结构受到严重破坏，从而导致DNA和蛋白质等胞内物质泄漏。UV/PAA处理能几乎完全抑制细菌暗修复现象，并且进一步灭活可存活但不可培养（VBNC）的细胞，表现出远远超过单独紫外线或过氧乙酸的氧化能力。此外，UV/PAA工艺还可以有效去除水中抗性基因（ARGs）。更多还原

【Abstract】 Peracetic acid(PAA) can effectively inactivate bacteria and control disinfection byproducts(DBPs). However, it may promote bacterial reactivation after treatment. This study investigated the performance and mechanism of the UV/PAA process for P. aeruginosa inactivation to enhance disinfection. Experimental and characterization methods such as inactivation, dark repair,membrane permeability analysis and electron microscopy scanning showed that the inactivation performance of the UV/PAA process was superior to that of UV or PAA alone due to the generation of free radicals and intracellular reactive oxygen species(ROS) in the UV/PAA system. The membrane permeability of P. aeruginosa increased over time when exposed to the UV/PAA system. Additionally,severe disruption of the cell surface structure was observed, resulting in the leakage of intracellular material such as DNA and proteins. The UV/PAA treatment was highly effective in inhibiting the bacterial dark repair phenomenon and further inactivating the viable but non-culturable(VBNC) cells,demonstrating a far superior oxidation ability of UV or PAA alone. Additionally, the UV/PAA process could efficiently eliminate antibiotic resistance genes(ARGs).更多还原