【摘要】 目的 探讨川芎挥发油对原发性痛经（PD）的治疗作用，并初步探讨其作用机制。方法 将48只雌性未孕KM小鼠随机分为空白组、模型组、阳性对照组和低、中、高剂量实验组。除空白组外，其余组均灌胃戊酸雌二醇构建PD小鼠模型。从第7天开始，上午空白组和模型组均灌胃给予等体积的蒸馏水，阳性对照组灌胃给予120 mg·kg^-1布洛芬溶液，低、中、高剂量实验组分别灌胃给予22、44、88 mg·kg^-1川芎挥发油溶液，连续5 d。用酶联免疫吸附试验法检测小鼠子宫组织中缩宫素（OT）和前列腺素F_2α(PGF_2α)含量。用缩宫素诱导的大鼠离体子宫收缩模型，通过不同阻断药（吲哚美辛、甲磺酸酚妥拉明、盐酸普萘洛尔、硫酸阿托品、盐酸苯海拉明、盐酸雷尼替丁和硝苯地平）预孵育的方式来探究川芎挥发油舒张子宫平滑肌作用机制。结果 空白组、模型组、阳性对照组和低、中、高剂量实验组的OT含量分别为（3.57±0.81）、（5.54±0.60）、（4.63±0.75）、（4.15±0.76）、（4.04±1.19）和（3.67±0.66）pg·mg^-1,PGF_2α含量分别为（1.22±0.38）、（2.53±0.45）、（1.84±0.52）、（1.30±0.31）、（1.21±0.35）和（0.48±0.15）pg·mg^-1。模型组的上述指标与低、中、高剂量实验组和空白组、阳性对照组相比，在统计学上差异均有统计学意义（P<0.01,P<0.05）。在预孵育吲哚美辛、甲磺酸酚妥拉明和硫酸阿托品后，川芎挥发油的舒张子宫平滑肌作用明显减弱；在盐酸普萘洛尔、盐酸苯海拉明、盐酸雷尼替代和硝苯地平预孵育后，川芎挥发油的舒张子宫平滑肌活性未发生明显变化。结论 川芎挥发油对PD具有显著的治疗作用，其机制可能与抑制环氧合酶、阻断α以及M受体有关。更多还原

【Abstract】 Objective To explore the effects of essential oil of Ligusticum chuanxiong （LCEO） on primary dysmenorrhea （PD） and preliminarily its mechanism of action.Methods Forty-eight female non-pregnant KM mice were randomly divided into blank group,model group,positive control group and experimental-L,-M,-H groups.Except for the blank group,the remaining groups were gavaged with estradiol valerate to establish the PD mouse model.Starting from the 7th day,the blank group and the model group were gavaged with equal volumes of distilled water in the morning,the positive control group was administered 120 mg·kg^-1of ibuprofen solution by gastric lavage,while the low,medium,and high dose experimental groups were gavaged with22,44 and 88 mg·kg^-1of LCEO,respectively,for 5 consecutive days.Enzyme-linked immunosorbent assay （ELISA） was used to measure the levels of oxytocin （OT） and prostaglandin F_2α(PGF_2α) in mice uterine.Using the oxytocin-induced rat isolated uterine contraction model,the study explored the mechanism of LCEO in relaxing uterine smooth muscle through preincubation with different blocking agents （Indomethacin,phentolamine mesylate,propranolol hydrochloride,atropine sulfate,diphenhydramine hydrochloride,ranitidine hydrochloride and nifedipine）.Results The OT contents in the blank group,model group,positive control group and experimental-L,-M,-H groups were （3.57±0.81）,（5.54±0.60）,（4.63±0.75）,（4.15±0.76）,（4.04±1.19） and （3.67±0.66） pg·mg^-1;the PGF_2αcontents were （1.22±0.38）,（2.53±0.45）,（1.84±0.52）,（1.30±0.31）,（1.21±0.35） and （0.48±0.15） pg·mg^-1,respectively.The above indexes in the model group were compared with the experimental-L,-M,-H and blank,positive control groups,there were statistically significant differences in all comparisons （P<0.01,P<0.05）.After preincubation with indomethacin,phentolamine mesylate,and atropine sulfate,the relaxant effect on uterine smooth muscle of LCEO was significantly weakened.After preincubation with propranolol hydrochloride,diphenhydramine hydrochloride,ranitidine hydrochloride and nifedipine,the relaxant effect on uterine smooth muscle of LCEO did not show any significant changes.Conclusion LCEO has a significant effect on primary dysmenorrhea,and its mechanism may be related to inhibiting cyclooxygenase and blocking receptors α and M.更多还原