【摘要】 为提高姜黄素的利用率并实现缓释，将其分散到淀粉溶液中获得核层纺丝液，以可降解的聚乙烯醇配制壳层纺丝液，采用同轴静电纺丝技术制备载姜黄素的核壳结构纳米纤维膜，并对纳米纤维膜的形态结构和缓释性能进行表征与测试。结果表明：纳米纤维表面光滑，具有明显的核壳结构；壳层流速直接影响纳米纤维的形态结构，纤维直径和壳层厚度随壳层流速的增加呈先增大后减小的趋势；核层姜黄素以无定形状态存在，核层和壳层组分间存在氢键作用；随着壳层流速增加，负载率减小，包封率先增大后减小，但并未影响姜黄素的有效释放；在初始阶段姜黄素的释放速率较大，24 h后趋于平缓，进入缓释阶段，且在24 h和96 h内的累积缓释率均随壳层流速的提高而增大，均为Fickian扩散。更多还原

【Abstract】 Objective Curcumin(Cur) has widely used in food and medical field by virtue of its proven antioxidant and anti-inflammatory action. Because of its hydrophobicity, low bioavailability, and instability, reasonable drug delivery systems are gaining growing attention. The biocompatible or biodegradable materials used to prepare drug delivery systems are recognized to be the safe and sustainable choice. In order to improve the utilization of Cur and achieve sustained release, the Cur-loaded nanofibrous membranes was developed via coaxial electrospinning technology, with Cur/starch(St) as a core layer and the polyvinyl alcohol(PVA) as the shell layer. Method The coaxial electrospinning technology was adopted to develop Cur-loaded nanofibrous membranes with core-shell structure. The natural antioxidants Cur was added to the biopolymer St solution to form a core layer spinning solution, and the degradable PVA was used as the shell layer spinning solution. The nanofibrous morphological structure, fiber diameter, shell thickness and sustained-release performance were regulated by the shell flow rate. The structures and properties of nanofibrous membranes were characterized by means of scanning electron microscope, transmission electron microscopy, infrared spectrometer, X-ray diffractor and UV spectrophotometer.Result The flow rate of shell solution during the coaxial electrospinning was adjusted to 0.4, 0.6, 0.8, 1.0 mL/h, and the corresponding average nanofiber diameter was 235.34, 266.18, 315.38, 293.63 nm, respectively. When the shell flow rate was 0.8 mL/h, the shell thickness was 108.42 nm, which was larger than that of the other groups. Therefore, the shell flow rate had an obvious effect on the morphological structure of the nanofibers from SEM and TEM, and all nanofibers were featured with core-shell structure and a smooth fiber surface. From the FT-IR and XRD, it was depicted that Cur-loaded core-shell nanofiber membranes were successfully prepared, Cur in the core layer was amorphous, and hydrogen bonding existed between the components of core and shell, which provided a structural basis for sustained release. With the increase of shell flow rate, the content of PVA in the fibers gradually increased, and the relative content of Cur and St in the core layer decreased. Therefore, the loading capacity decreased with the increase of shell flow rate. However, the encapsulation efficiency first increased and then decreased when the shell flow rate increased. When the shell flow rate increased to 0.8 mL/h, the encapsulation efficiency increased to 71.11%. When the shell flow rate further increased to 1.0 mL/h, the encapsulation efficiency showed a slight decreasing to 68.26%, because a negative effect on the Taylor cone shape and the formation of fiber occurred when the shell flow velocity exceeded a certain value, which in turn affected the encapsulation effect of the nanofibers. The Cur release rate was high in the initial stage, and the release curve flattened after 24 h, entering the sustained release stage. With the increase of shell flow rate, the cumulative sustained release rate within 24 h and 96 h increased accordingly, realizing the regulation of Cur content and sustained-release effect. And the release mode exhibited the Fickian diffusion behavior.Conclusion The Cur-loaded nanofibrous membranes with core-shell structure were developed by the coaxial electrospinning technology. The Cur-loaded nanofiber had an obvious core-shell structure and a smooth fiber surface. The shell flow rate directly affected Cur content and morphological structure of nanofibers. With the increase of the shell flow rate, the fiber diameter and shell thickness first increased and then decreased. Cur in the core layer was amorphous, and hydrogen bonding existed between the components of corer and shell layer. With the increase of shell flow rate, the loading capacity decreased, and the encapsulation efficiency first increased and then decreased, which did not affect the efficient release of Cur. The shell flow rate influenced Cur content and cumulative release rate, and the release behaviors followed the Fickian diffusion law, which indicated the shell flow rate could regulate Cur content and sustained release effect. The Cur-loaded nanofiber membrane prepared in this paper has a broad application prospect in the food and medical fields.更多还原