【摘要】 目的 建立一种基于比率荧光探针的氰化物快速分析方法，为氰化物的现场可视化快速检测提供定量策略。方法 以牛血清白蛋白稳定的金纳米团簇（gold nanoclusters,AuNCs；荧光发射波长为660 nm）作为响应单元，荧光素（fluorescein,FL；发射波长为515 nm）作为内参，构建金纳米团簇与荧光素（AuNCs-FL）双发射比率荧光探针。结果 氰化物对AuNCs的刻蚀作用导致660 nm处荧光淬灭，而FL在515 nm处荧光信号保持不变，通过红色到绿色的荧光变化实现氰化物的快速分析。该荧光探针可在3 min内完成检测，检出限为3.4 mg/L，可视化检测范围为10～100 mg/L。结论 AuNCs-FL荧光探针组成简单、成本较低，操作简单且快速准确，可避免氰化物检测试剂盒易受硫化物干扰的问题，能够满足氰化物中毒案件中可疑粉末检材的现场快速检测。更多还原

【Abstract】 Objective To establish a rapid analysis method for cyanide based on a ratiometric fluorescent probe, providing a quantitative strategy for on-site visual and rapid detection of cyanide. Methods A dual-emission ratiometric fluorescent probe(AuNCs-FL) was constructed by using bovine serum albumin(BSA)-stabilized gold nanoclusters(AuNCs, fluorescence emission at 660 nm) as the responsive signal unit and fluorescein(FL, emission at 515 nm) as the internal reference. Results The etching effect of cyanide on AuNCs resulted in fluorescence quenching at 660 nm, while the fluorescence intensity of FL at 515 nm remained unchanged, enabling a rapid response analysis of cyanide shift from red to green fluorescence. The developed probe enabled rapid analysis of cyanide within 3 min, with a limit of detection(LOD) of 3.4 mg/L and a visual detection range of 10-100 mg/L. Conclusion The AuNCs-FL fluorescent probe is structurally simple, low-cost, and easy to operate, delivering rapid and accurate results. It also avoids the interference from sulfides encountered in commercial cyanide test kits, making it suitable for the on-site rapid detection of suspected powder samples in cyanide poisoning cases.更多还原