【摘要】 研究开发了HPLC法同时测定香菊胶囊中木兰脂素、5-O-甲基维斯阿米醇苷、迷迭香酸、木兰脂素成分含量测定的方法。Waters symmetry C18色谱柱分离，流动相为乙腈（A）和0.1%磷酸（B），梯度洗脱，流速为1.0mL/min，采集波长：迷迭香酸（331nm）、木兰脂素（278nm）、蒙花苷（335nm）和5-O-甲基维斯阿米醇苷（255nm），柱温为室温，进样量为10μL,HPLC-DAD定量检测。结果表明，迷迭香酸在0.4905～49.5μg/mL、木兰脂素在0.09840～9.840μg/mL、蒙花苷在0.02365～2.365μg/mL和5-O-甲基维斯阿米醇苷在0.05868～5.868μg/mL范围内，均有良好的线性，平均回收率分别为99.60%、99.32%、100.0%、99.62%；仪器精密度和方法重复性RSD均在5.0%以内。此方法具有前处理简单等优点，适用于香菊胶囊成品的质量监控。更多还原

【Abstract】 A method for simultaneous determination of magnolol, 5-O-methylvisamminol glycoside, rosmarinic acid, and magnolol components in Xiangju capsules by HPLC was developed. Waters symmetry C18 column was used for seperation. The mobile phase was consisted of acetonitrile(A) and 0.1% phosphoric acid solution(B) with gradient elution. The column flow rate was 1.0 mL/min. The detection wavelengths of rosmarinic acid, magnolitin, montanin and 5-O-methylvisamitol side were set to 331 nm、278 nm、335 nm and 255 nm, respectively. The injection volume was 10μL. Quantitative detection by HPLC-DAD Rosmarinic acid, magnolitin, montanin and 5-O-methylvisamitol side showed good linear relationships in concentration range of 0.4905-49.5μg/mL、0.09840-9.840、0.02365-2.365、0.05868-5.868μg/mL. The average recoveries were 99.60%、99.32%、100.0%and 99.62%, respectively. Precision and reproducibility of the method were all within 5.0%. This method has the advantages of simple pretreatment, fast speed and accurate result, etc.更多还原