【摘要】 建立了高效液相色谱法测定预制菜中3种防腐剂含量的方法。采用Waters ZORBAX RX-C18色谱柱（250mm×4.6mm,5μm）为分离柱，以甲醇-0.02mol/L乙酸铵（体积比为8∶92）为流动相，流量为1.0mL/min，进样体积为10μL，柱温为30℃，检测波长为230nm和293nm。3种防腐剂的质量浓度在0.1～100mg/L范围内与色谱峰面积线性关系良好，线性相关系数均大于0.9999，方法检出限范围在0.006g/kg，定量限为0.02g/kg。测定结果的相对标准偏差为1.05%～3.16%(n=7)，样品加标平均回收率为86.1%～104.6%。该方法的精密度和准确度良好，可用于预制菜中对3种防腐剂含量的测定。更多还原

【Abstract】 A method for the determination of benzoic acid, sorbic acid and dehydroacetic acid in pre-made food by high performance liquid chromatography was established. The Waters ZORBAX RX-C18 column(250mm×4.6mm, 5μm) was used as separation column, the mobile phase was a mixture of methanol and 0.02mol/L ammonium acetate(volume ratio was 8:92), and the flow rate was 1.0mL/min. The detection wavelength was 230nm and 293nm.The concentration of preservatives had a good linear relationship with the chromatographic peak area in the range of 0.1-100mg/L, the correlation coefficient were great than 0.9999. The detection limit of the method were 0.006g/kg,and the quantification limit was 0.02g/kg. The average recovery of sample spiked were 86.1%-104.6%, and the relative standard deviation of the determination results were 1.05%-3.16%. The method is applicable for the determination of the three preservatives′ content in pre-made food.更多还原