【摘要】 荧光作为一种经典的物理化学现象，在揭示生命科学的深层次机制和推动精准医疗革命方面发挥了重要作用。旨在介绍荧光成像技术在生物医学研究中的尖端应用，从自然界中的荧光现象出发，讨论了荧光产生的机理，荧光染料的发展历程，多功能荧光探针的设计策略，以及荧光成像技术在活细胞探测、疾病检测、光学治疗和术中导航中的先进应用，深入浅出地对荧光相关的成像技术做了科普介绍，希望为化学、材料学、生物学和医学等多个学科的科研工作者或对此感兴趣的人员提供关于荧光成像技术的核心概念、当前热点和最新进展知识。更多还原

【Abstract】 Fluorescence, as a classic phenomenon in physical chemistry, has played a significant role in uncovering the deep mechanisms of life sciences and driving the revolution in precision medicine.This paper aims to introduce the cutting-edge applications of fluorescence imaging technology in biomedical research.Starting from the natural phenomena of fluorescence, it discusses the mechanisms of fluorescence generation, the development history of fluorescent dyes, the design strategies of multifunctional fluorescent probes, and the advanced applications of fluorescence imaging technology in live cell detection, disease diagnosis, phototherapy, and intraoperative navigation.By providing a comprehensive and accessible overview of fluorescence-related imaging technology, this paper seeks to offer core concepts, current hot topics, and the latest advancements in fluorescence imaging technology to researchers and interested individuals in chemistry, materials science, biology, medicine, and other disciplines.更多还原