【摘要】 【目的】探明深色有隔内生真菌（dark septate endophytes, DSE）对番茄生物学特性的影响，并筛选出对番茄具有促生和抗灰霉病作用的DSE菌株，为肥药减量化施用、改善农产品质量提供可行的解决思路。【方法】以8株DSE菌株和灰葡萄孢为研究对象，用共培养和平板对峙培养法比较DSE对番茄的促生作用及其对灰葡萄孢的抑制作用。【结果】比较8株DSE菌株对番茄苗生物量的影响，结果表明Xylaria berteroi(BND 6-R1-13)显著促进番茄生长，提高叶片色素含量，促进根系生长（侧根数、根冠比）和根活力，对灰葡萄孢菌丝生长抑制率为44.8%;Cordyceps javanica(BND 5-R1-3)显著促进番茄侧根数、根活力和花青素含量。Pantospora guazumae(BND 5-L2-3)对灰葡萄孢菌丝生长抑制率为73.5%，与菌株BND 6-R1-13一样是对灰葡萄孢有抑制作用的DSE菌株。【结论】首次报道了对灰葡萄孢有抑制作用的两个菌株X. berteroi(BND 6-R1-13)和P. guazumae(BND 5-L2-3)，其中X.berteroi(BND 6-R1-13)与番茄共培养生长15 d后，显著促进番茄幼苗的生长，并能够抑制灰葡萄孢的菌落生长，具有潜在的促生和抗病作用。更多还原

【Abstract】 【Objective】 Dark septate endophytes(DSEs) were screened for growth promotion and disease resistance to gray mold of tomato plants. 【Methods】 An experimentation using 8 species of DSEs cultured on PDA plates along with the gray mold, Botrytis cinerea Pers. Fr, was conducted to examine the inhibitory effects of the DSEs on the pathogen. On a MS medium, growth indicators of tomato seedlings germinated from seeds with the DSEs were monitored. Infection rates of the tomato seedlings in the co-culture were observed.【Results】 Of the 8 DSEs, Xylaria berteroi(BND 6-R1-13) significantly enhanced the seedling growth, increased the leaf pigments content, boosted the root development(as shown on the increased lateral root number and root/shoot ratio), and heightened the root activity. It significantly inhibited the B. cinerea colony proliferation, achieving a control efficacy of 44.8%. Another DSE, Cordyceps javanica(BND 5-R1-3) significantly increased the lateral root count, root activity, and anthocyanin content of the tomato seedlings. And a third one, Pantospora guazumae(BND 5-L2-3) significantly inhibited the growth of B. cinerea showing a control efficacy of 73.5%. 【Conclusion】 For the first time, the significant inhibitory effects of X. berteroi(BND 6-R1-13) and P. guazumae(BND 5-L2-3) on B. cinerea were reported by this study. In co-culturing with X. berteroi(BND 6-R1-13) for 15 d, the growth of the tomato seedlings was significantly enhanced. In addition, with the significant inhibitory effect on B. cinerea, the DSE would warrant further investigation for tomato farming application.更多还原